Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 268-612-2
CAS number: 68131-30-6
A solution obtained by dissolving the chemicals recovered in the alkaline pulping process in water.
study was performed to determine a possible mutagenic action of "Green
liquor 1" with
the Salmonella typhimurium reverse mutation test (Ames test). This test
is sensitive to frameshift mutations as well as to base pair mutations.
The performance of the test with and without an external metabolising
system (a lyophilised
post-mitochondrial supernatant of homogenised livers of male Sprague
Dawley rats, induced with Aroclor 1254, S9
-Mix) enables the detection of the mutagenic action of the test
substance itself as well as of its metabolites.
strains of Salmonella typhimurium TA97a, TA98, TA100, TA102 and TA1535
were used. The exposure technique was the plate incorporation method in
the first experiment and the preincubation method in the second
experiment. The following concentrations were tested: 5000, 1667, 556,
185 and 62 µg/plate.
toxicity of the test substance to the bacteria was observed up to
5000 µg per plate in both experiments.
none of the concentrations tested and with none of the strains used an
increase of the mutation frequency to more than the threshold values
(250 % of the controls for strains TA98 and TA1535, 167 % of the
controls for strains TA97a, TA100 and TA102) was obtained. Metabolic
activation did not change these results.
to the results obtained in this study the test item is non-mutagenic
in the Ames test with
the strains TA97a, TA98, TA100, TA102 and TA1535 with and without an
external metabolising system up to 5000 µg/plate, which is the limit
concentration for this kind of test.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again