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EC number: 273-606-8 | CAS number: 68990-52-3
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- November 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study not in GLP, purity of the sample has not been assessed, but the study is conducting following official guideline and scientifically valid to assess the end point
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- yes
- Remarks:
- Water accomodation fraction
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Solutions are prepared just before testing. In comparaison with the control, a series of geometric concentration solutions has been prepared to obtain 100% imobilisation after 48h.
WAF solutions are prepared: 10%, 1%, 0.1%, 0.01%, 0.001% - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna strauss of more than 6 hours and less than 24 hours old.
Source: U.G. University of Gand - lab. Prof Persoone
Alimentation: algae Selenastrum capricornutum - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No
- Test temperature:
- 20°C +/- 1°C
- Nominal and measured concentrations:
- 10%, 1%, 0,1%, 0.01%, 0.001%
- Details on test conditions:
- Total obscuration
Not air, light or feeding during the test - Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 2 504 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- not specified
- Conclusions:
- WAF EC50 48 Daphnia magna = 2504 mg/l
- Executive summary:
The short term toxicity to aquatic invertebrates has been assessed following OECD 202 on rape oil methyl ester, with deviation for poor soluble substances following ISO 147.
Reference
20 organismas for any concentration divided in 4 goups of 5 animals each
Results table:
| WAF concentration | Imobilised daphnis after 48h | % imobilised | |||
| A | B | C | D | ||
| Control | 0/5 | 0/5 | 0/5 | 0/5 | 0 |
| WAF 10% | 5/5 | 5/5 | 5/5 | 5/5 | 100 |
| WAF 1% | 4/5 | 5/5 | 4/5 | 5/5 | 80 |
| WAF 0.1% | 1/5 | 0/5 | 2/5 | 0/5 | 15 |
| WAF 0.01% | 0/5 | 0/5 | 0/5 | 0/5 | 0 |
| WAF 0.001% | 0/5 | 0/5 | 0/5 | 0/5 | 0 |
Description of key information
WAF EC50 = 2504 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 2 504 mg/L
Additional information
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