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EC number: 273-606-8 | CAS number: 68990-52-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994-09-13 to 1995-04-21
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The study follows the 301B OECD guideline, GLP procedures and all validity criteria are fulfilled. The test solution is prepared via a volatil solvent but no solvent control was performed The test solution is slightly heated (at 40°C) before the beginning of the test but there is no information of the impact of this warming on the stability of esterol A and hence on its biodegradability in the test conditions.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- yes
- Remarks:
- for details see material and methods freetext
- GLP compliance:
- yes
- Remarks:
- acreditation number 1-0210 (COFRAC)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: Secondary effluent from a biologic treatment plant
- Details on inoculum:
- - Type of sludge: other: Secondary effluent from a biologic treatment plant (domestic sewage)
- Sampling site: Versailles (France, 78)
- Preparation of inoculum: The effluent sample was collected the day before the test. Then it was centrifugated at 20°C during 20 min at 4000g and the pellet resuspended in test medium to reach a concentration factor of 40 in comparison to initial effluent sample.
- Initial cell concentration (after concentration treatment): 2.7 10E4 bact/mL
- cell concentration after preparation: 2.7 10E2 bact/mL
- Pretreatment: aeration until inoculation
- adaptation: no data is given about an eventual adaptation of he inoculum to the test solution, therefore it is considered by default that the inoculum had not been pre-adapted. - Duration of test (contact time):
- 29 d
- Initial conc.:
- 20 mg/L
- Based on:
- other: TOC
- Initial conc.:
- 25.7 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST SYSTEM
- Culturing apparatus: 3-litre Erlenmeyer flasks.
- Number of culture flasks per concentration: 2.
- Aeration device: Bubbling CO2-free air in darkness.
The CO2 elimination process is materialized by the aeration of flasks with CO2-free air after addition of the substance and before introducing
inoculum.
- Measuring equipment: Jenway 3410, Maïhak Tocor 100.
- Closed vessels used: Yes.
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 flasks (inoculum)
- Abiotic sterile control: yes (test sub., inoculum and sterilising agent)
- Surface active agent control: 1 flask (emulsion of surface active agent, and inoculum)
- Toxicity control: 1 flask (test sub. reference sub., and inoculum)
- Procedure control: 1 flask (ref. sub. and inoculum)
METHOD OF PREPARATION OF TEST SOLUTION:
The substance was dissolved in a water-miscible and very volatile solvent:the Dichloro-1,1-fluoro-1-ethane.
The substance emulsion was realised by the addition of Synperonic P94 (surface active agent not biodegradable nor toxic).
This mixture was heated up to 40°C in order to facilitate the evaporation of the solvent.
ANALYTICAL PARAMETER:
Quantitative analysis of CO2 released.
The CO2 produced was trapped in barium hydroxide (0.0125M) and measured by titration of the residual hydroxide.
SAMPLING: 0, 1, 4, 5, 8, 11, 13, 18, 21, 28, 29 days.
TEST CONDITIONS
- Composition of medium: (same as requested in the guideline)
For 1 litre:
1) 10 ml of solution a:
8.5g KH2PO4
21.75g K2HPO4
33.4 g Na2HPO4,H2O
0.5g NH4Cl
ultrapure water up to 1000ml .
2) 1 ml of solution b: 27.5g CaCl2 or 36.40g CaCl2,2H2O in 1000 ml of ultrapure water.
3) 1 ml of solution c: 22.5g MgSO4,7H2O in 1000 ml of ultrapure water.
4 ) 1 ml of solution d: 0.25g FeCl3,6H2O in 1000 ml of ultrapure water (this solution was prepared at the beginning of the test).
5 ) 800 ml of pure water.
6 ) ultrapure water up to 1000 ml.
- Additional substrate: No.
- Test temperature: 22+/-2°C. - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 75
- Sampling time:
- 29 d
- Details on results:
- - 65% biodegradation in the 10-d window (see figure 1 in document attached: EsterolA_5.1.2_Thiebaud1995_fig1 kinetic of degradation.doc)
- procedure control: 90% biodegraded after 29 days
- surface active agent control similar to blank
- No inhibition of inoculum observed.
- The degradation of the substance is only attributed to micro-organism. No physico-chemical degradation can be attributed to the degradation of the Esterol A.
- Results with reference substance:
- see table 1 kinetic of degradation
- Validity criteria fulfilled:
- yes
- Remarks:
- for details see freetext overall remarks
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The maximum biodegradation rate of Esterol A is 75% in 28d. Esterol A is readily biodegradable.
- Executive summary:
The study of the ready biodegradability of Esterol A according to OECD 301B guideline, show a maximum level of biodegradation of 75% in 28days.
The percentage of biodegradation at the end of the 10-d window is 65%.
In the test conditions, no inhibitory effect of Esterol A on the micro-organism of the inoculum nor abiotic degradation were observed and all validity criteria were fulfilled.
Esterol A is considered as readily biodegradable.
Reference
Degradation products: not measured. No effect of the surface active agent added in the test suspension Table 1: Kinetics of degradation (in %) of Esterol A (test substance), benzoic acid (ref substance) and toxicity control (EsterolA + benzoic acid)
Day |
0 |
1 |
4 |
5 |
8 |
11 |
13 |
18 |
21 |
28 |
29 |
Test suspension 1 |
0 |
0 |
36 |
48 |
60 |
66 |
69 |
74 |
76 |
79 |
79 |
Test suspension 2 |
0 |
0 |
35 |
45 |
56 |
61 |
63 |
68 |
70 |
71 |
71 |
Test suspension Average |
0 |
0 |
36 |
46 |
58 |
63 |
66 |
71 |
73 |
75 |
75 |
Inoculum blank |
0 |
20 |
68 |
72 |
78 |
83 |
84 |
86 |
87 |
88 |
90 |
Toxicity control |
0 |
2 |
39 |
49 |
61 |
66 |
68 |
72 |
74 |
76 |
76 |
Description of key information
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
Biodegradability in water has been assessed on the registering substance: 75% in 28 days with 60% in the 10 -day windows, half life of 6/7 days
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