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Long-term toxicity to fish

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The long-term toxicity for fish endpoint is addressed by read across to long-term toxicity for aquatic invertebrates (Daphnia magna). Read across to OLBOs has been applied for the long-term toxicity to aquatic invertebrates endpoint, which is justified based on similarities in composition and physical/chemical properties between highly refined base oils and OLBOs. Toxic effects of hydrocarbons are primarily caused by narcosis, and occur in a narrow range of molar concentrations across aquatic taxa; hence, read across between species is justified.

Results of computer modelling to estimate aquatic chronic toxicity in a 28-day freshwater fish study show no chronic toxicity of highly refined base oil to freshwater fish at or below its maximum attainable water solubility. This supports the applied interspecies read across (Redman et al., 2010b).