Registration Dossier
Registration Dossier
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EC number: 203-632-7 | CAS number: 108-95-2
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
The inhibition of dehydrogenase activity and the inhibition of microbial nitrification were tested in the presence of phenol at concentrations of 1, 10, 100, 1000 mg/kg soil dry weight. As the applied enzymatic activities are used for the assessment of pesticides, it is assumed that the standarized method for the testing for pesticides was applied. In contrast to the standardized method, the incubation period was 14 days (guideline: at least 28 days). Lowest endpoint was the EC10 for dehydrogenase activity (100 mg/kg soil dry weight).
Key value for chemical safety assessment
- Long-term EC10 or NOEC for soil microorganisms:
- 100 mg/kg soil dw
Additional information
The data on effects of phenol on terrestrial soil organisms is presented in EU RAR (2006) in Section 3.2.3 (Terrestrial compartment, page 44ff).
It is stated that the most sensitive endpoint for soil microorganisms is the NOEC > 100 mg/kg dry weight obtained for reduction of hydrogenase activity (content of organic matter not stated; Maas & Auspurg, 1984).
The inhibition of dehydrogenase activity and the inhibition of microbial nitrification were tested in the presence of phenol at concentrations of 1, 10, 100, 1000 mg/kg soil dry weight. The applied soil and further test conditions are not described. At the same time several pesticides were tested. As the applied enzymatic activities are used for the assessment of pesticides, it is assumed that the standardized method for the testing for pesticides was applied. In contrast to the standardized method, the incubation period was 14 days (guideline: at least 28 days).
Based on the percentual deviation of dehydrogenase activity from the untreated control samples, it can be estimated that the EC10 for dehydrogenase activity is 100 mg/kg soil dry weight. Based on the nitrogen concentrations, shown in a diagram, it can be concluded that the EC50 for nitrification is in the range of 100 - 1000 mg/kg soil dry weight.
As it is assumed that the standard test for the testing of chemicals and pesticides were used and as it is not expected that further tests will result in completely different results, no further tests are considered to be necessary.
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