Turpentine, oil

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1972

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1972

Report date:

1972

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Turpentine, oil

EC Number:

232-350-7

EC Name:

Turpentine, oil

Cas Number:

8006-64-2

Molecular formula:

UVCB substance molecular formula varied but mainly C10H16, C15H24, C2H6S1, C1H4S1 and C10H18O1

IUPAC Name:

Turpentine oil from pulping processes

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

No details available.

Administration / exposure

Route of administration:

oral: unspecified

Vehicle:

water

Details on oral exposure:

No details available

Doses:

3.2, 4.0. 5.0 and 6.25 ml/kg

No. of animals per sex per dose:

10M

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days

- Frequency of observations and weighing: 1 and 6 hours after dosing, once per day thereafter.

- Necropsy of survivors performed: yes

Statistics:

Method not stated

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

4.6 mL/kg bw

Based on:

test mat.

95% CL:

3.9 - 5.3

Mortality:

See Table 1

Clinical signs:

other: Slight ataxia and lethargy.

Gross pathology:

There were no abnormalities in any animals at necropsy.

Other findings:

None reported.

Any other information on results incl. tables

The LD₅₀ of the compound calculated from the data obtained is 4.6 ml/kg with 95% confidence limits of 3.9 ml/kg and 5.3 ml/kg. No abnormalities were noted in any of the animals at necropsy. Doses and number of deaths were:

Dose (ml/kg)	Deaths
3.2	1/10
4	4/10
5	6/10
6.25	8/10

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

In an acute oral toxicity study with male Wistar rats, the LD50 was determined to be 4.6 ml/kg.