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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non GLP, near guideline study, available as unpublished report, restrictions in design and/or reporting but otherwise adequate for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1982
Report date:
1982

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
GLP compliance:
no
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Cyclohexane
EC Number:
203-806-2
EC Name:
Cyclohexane
Cas Number:
110-82-7
Molecular formula:
C6H12
IUPAC Name:
cyclohexane
Details on test material:
- Name of test material (as cited in study report): cyclohexane
No further details reported

Test animals

Species:
rat
Strain:
not specified
Sex:
male/female
Details on test animals or test system and environmental conditions:
No details reported

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: air
Details on inhalation exposure:
No details reported
Analytical verification of test atmosphere concentrations:
yes
Duration of exposure:
4 h
Concentrations:
Nominal concentration 32.88 mg/L (9570.43 ppm)
Mean analytical concentration 21250.0±925.82 ppm
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Observations recorded once per hour during exposure and once per day thereafter. Bodyweights recorded pre-exposure and on days 2, 3, 4, 7 and 14 post-exposure.
- Necropsy of survivors performed: yes
Statistics:
Not required, limit dose

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 32 880 mg/m³ air
Exp. duration:
4 h
Remarks on result:
other: no mortalities
Mortality:
No mortalities
Clinical signs:
other: During exposure, observations in males and females included tremors, hyperactivity, rapid respiration and lying prostrate in cage. During the post-exposure period all females appeared normal, one male had localised alopecia up to day 10 and sores around t
Body weight:
No exposure-related effects
Gross pathology:
No exposure-related effects

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The acute inhalation LC50 of cyclohexane to male and female rats, following a 4 hour exposure, was >32.88 mg/L.
Executive summary:

The acute inhalation LC50 of cyclohexane to male and female rats, following a 4 hour exposure, was >32.88 mg/L.