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EC number: 232-379-5 | CAS number: 8011-76-5 Substance obtained by treating phosphate rock with sulfuric acid or a mixture of sulfuric and phosphoric acids. Composed primarily of calcium phosphates and calcium sulfate.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian germ cell study: cytogenicity / chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- disregarded due to major methodological deficiencies
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- significant methodological deficiencies
- Remarks:
- - The dose applied is extremely high (if body weight mouse is 25 g: 20,000 mg/kg bw/d). This is 10 times the maximum dose allowed according to OECD 475. - The number of animals used is not mentioned and individual data are not given (it could be possible that 3 animals have been used and the deviation was only in one animal). - The number of metaphases is 300, while 100 per animal on a group of 5 animals per dose are required according to OECD 475. - No positive control used.
Data source
Reference
- Reference Type:
- publication
- Title:
- Induction of mitotic-chromosome anomalies in mice by single super phosphate.
- Author:
- Chaurasia, O.P. and Sinha, S.P.
- Year:
- 1 988
- Bibliographic source:
- Cytologia 53:485-489.
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- GLP compliance:
- not specified
- Type of assay:
- mammalian bone marrow chromosome aberration test
Test material
- Reference substance name:
- Superphosphates
- EC Number:
- 232-379-5
- EC Name:
- Superphosphates
- Cas Number:
- 8011-76-5
- Molecular formula:
- not applicable
- IUPAC Name:
- Superphosphates
- Test material form:
- solid: particulate/powder
- Details on test material:
- No additional data available.
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- Swiss
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- 7-10 weeks old
Administration / exposure
- Route of administration:
- oral: feed
- Details on exposure:
- Single superphosphate was fed to 7-10 week old mice for seven days at a rate of 500 mg/day after mixing with basic food.
- Duration of treatment / exposure:
- 7 d
Doses / concentrations
- Dose / conc.:
- 500 mg/kg bw/day (nominal)
- No. of animals per sex per dose:
- No data
- Control animals:
- no
- Positive control(s):
- No data
Examinations
- Tissues and cell types examined:
- Their bone marrow was separated from both femura and slides were prepared. Screening of any chromosomal abnormalities was made in 300 well spread metaphase plates.
- Details of tissue and slide preparation:
- Of the 300 metaphase plates, 90 abnormal ones were found in treated animals in contrast to 15 among the controls, a six-fold increase in chromosomal abnormalities. Among the gross types, clumping and stickiness of chromosomes as well as pulverization were observed.
Results and discussion
- Additional information on results:
- Among the individual types, the breaks were in the form of subchromatidal and chromatidal nature. Translocations, gaps, and constrictions were also observed. The frequency of chromosomal abnormality relative to controls increased by 40% whereas that of gross and individual types was by 5 and 35%, respectively. The breaking-points of the affected chromosomes were somewhat localized in the distal region. According to the authors, single superphosphate, when administered to mammals, is clastogenic and appears to be mutagenic.
Applicant's summary and conclusion
- Conclusions:
- Due to major methodological deficiencies the study cannot be used for classification.
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