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Toxicity to aquatic algae and cyanobacteria

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Key value for chemical safety assessment

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In a key, static 72 hourPseudokirchneriella subcapitatagrowth inhibition test (OECD 201, KS=1), 10000 cells/mL were exposed to the Water Accommodated Fraction (WAF) of heavy cracked fuel oils (MRD-10-579) at nominal concentrations of 0.05, 0.23, 1.01, 4.56 and 20.5 mg/L. The 72 hour EL50 was 0.32 mg/L and NOELR was 0.05 mg/L based on growth rate (EMBSI 2012b).

In seven reliable supporting 72-hour algae (Pseudokirchnerella subcapitata)toxicity studies using WAF methodology, the EL50 values based on growth rate and biomass varied between 0.75 to >107 mg/L and 0 - <300mg/L, respectively (EMBSI, 2008j,k,l,m; Shell, 1997a, b).