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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Reference
Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Principles of method if other than guideline:
Effects were evaluated following repeated topical exposure
GLP compliance:
not specified
Test organisms (species):
Lumbricus sp.
Animal group:
nematods
Details on test organisms:
TEST ORGANISM
- Common name: Earthworm
- Age at test initiation: sexually mature
- Weight at test initiation: 4-5 g

ACCLIMATION
- Acclimation period: one week
- Acclimation conditions: held in a medium consisting of nonsterilized potting soil fortified with cornmeal at 13°C
Study type:
laboratory study
Substrate type:
artificial soil
Post exposure observation period:
21 days.
Test temperature:
13 degree C after treatment occurred.
Details on test conditions:
TEST SYSTEM
- Topical application: Instrumentation Specialties microapplicator with a 1.0-ml Hamilton Syringe fitted with a 27-gauge needle.
- Volume: 1-4 ul
- Area of application: dorsum, anterior to the clitellum.

OTHER TEST CONDITIONS
- Light intensity: dark

EFFECT PARAMETERS MEASURED: criteria for a toxic response included weight loss, appearance of sores or swellings, coiling, and mortality.

Duration:
21 d
Dose descriptor:
other: Percentage mortality
Effect conc.:
156 - 780 other: mg/kg (applied topically to earthworms)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other:
Remarks:
Acetonitrile caused no irritation or mortality when applied topically to earthworms at doses estimated to be in the range of 156 mg/kg to 780 mg/kg over 21 days.

Toxicity of DDT to the Earthworm via Topical Application in Acetonitrile

     % Mortality   % Mortality   % Mortality   % Mortality
 Dose  Solvent  24 h  7 d  14 d  21 d
200  Acetonitrile 10 20 20 20
100 Acetonitrile 10 20 20 20
50 Acetonitrile 0 0 0 0
25 Acetonitrile 10 20 20 20
5 Acetonitrile 0 10 10 10
0 Acetonitrile 0 0 0 0
Conclusions:
Scrutiny of several different solvents revealed that acetonitrile caused no irritation when applied topically to the earthworm
Executive summary:

Fisher SW (1984) reported no evidence of irritation or mortality (21 days) in earthworms treated with acetonitrile by topical application. Acetonitrile was a solvent control used in this study. Doses of 1 -4 ul were applied topically to worms weighing 4 -5 g. This corresponds to doses in the range of 156 mg/kg to 780 mg/kg.

Description of key information

Fisher SW (1984) reported no evidence of irritation or mortality (21 days) in earthworms treated by topical application to acetonitrile doses estimated to be in the range of 156 mg/kg to 780 mg/kg.

Key value for chemical safety assessment

Additional information

Fisher SW (1984) reported no evidence of irritation or mortality (21 days) in earthworms treated with acetonitrile by topical application. Acetonitrile was a solvent control used in this study. Doses of 1 -4 ul were applied topically to worms weighing 4 -5 g. This corresponds to doses in the range of 156 mg/kg to 780 mg/kg.