Acetonitrile

Long-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to fish, other

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Sampling and measured concentration: 0, 7d, 14d, 21d
- Sampling volume: ca. 2 mL

Vehicle:

no

Details on test solutions:

A series of stock solutions (14000, 26000, 50000 mg/L) were prepared by dissolving the test substance in the purified water.

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Orange killifish
- Source: Niikura fish farm
- Length at study initiation: 2.2 cm (2.0 - 2.3 cm), n=10
- Weight at study initiation: 0.16 g (0.11 - 0.23 g), n=10
- Feeding during test
- Food type: TetraMin
- Amount: 2%/fish weight
- Frequency: daily


ACCLIMATION
- Acclimation period: 54 days
- Acclimation conditions: Same as test
- Type and amount of food: TetraMin

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test temperature:

23.7 - 24.0℃

pH:

7.4 - 7.9

Dissolved oxygen:

5.5 - 8.2 mg/L

Nominal and measured concentrations:

nominal concentration: 29, 53, 102 mg/L (and control)
mean measured concentration: 28, 53, 96 mg/L (and control)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material: glass beaker
- Size: 5.0 L
- Aeration: No
- Type of flow-through: proportional pump
- Renewal rate of test solution: 24.45 mL/min
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Hardness of dilution water: 50 mg/L (as CaCO3)
- pH of dilution water: 7.9

OTHER TEST CONDITIONS
- Photoperiod: 16h light/ 8h dark

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: acute toxicity study (96h); 100 mg/L
- Results used to determine the conditions for the definitive study: 96h-LC50 > 100 mg/L

Reference substance (positive control):

yes

Remarks:

Copper(Ⅱ) sulfate pentahydrate

Duration:

7 d

Dose descriptor:

LC50

Effect conc.:

> 102 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 102 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

> 102 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

> 102 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: mortality, toxicological symptoms, body weight, body length,

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

102 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: mortality, toxicological symptoms, body weight, body length,

Details on results:

- Observations on body length and weight: No difference
- Mortality of control: 0

Results with reference substance (positive control):

- 96h LC50: 0.88 mg/L

Reported statistics and error estimates:

Fish weight and length: Dunnett's multiple comparison test, Two-tailed test (α=0.05)

1. Method OECD Guidelines for Testing of Chemicals "Fish, Prolonged Toxicity Test: 14 -day Study (Guideline 204, 1984)"              

2. Test organisms Oryzias latipes

3. Result 7d LC50>102 mg/L (Nominal concentration) 14d LC50>102 mg/L (Nominal concentration) 21d LC50 >102 mg/L (Nominal concentration) 21d LOEC >102 mg/L (Nominal concentration) 21d NOEC = 102 mg/L (Nominal concentration)

Executive summary:

In a guideline (OECD 204) and GLP study, MCSI of Japan (1996) reported the 21 -day NOEC for acetonitrile in fish (Oryzias latipes) to be 102 mg/L (nominal conc.) using flow-through exposure conditions.

Description of key information

In a guideline (OECD 204) and GLP study, MCSI of Japan (1996) reported the 21-day NOEC for acetonitrile in fish (Oryzias latipes) to be 102 mg/L (nominal conc.) using flow-through exposure conditions.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

102 mg/L

Additional information

In a guideline (OECD 204) and GLP study, MCSI of Japan (1996) reported the 21 -day NOEC for acetonitrile in fish (Oryzias latipes) to be 102 mg/L (nominal conc.) using flow-through exposure conditions.