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EC number: 200-835-2 | CAS number: 75-05-8
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to fish, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling and measured concentration: 0, 7d, 14d, 21d
- Sampling volume: ca. 2 mL - Vehicle:
- no
- Details on test solutions:
- A series of stock solutions (14000, 26000, 50000 mg/L) were prepared by dissolving the test substance in the purified water.
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- TEST ORGANISM
- Common name: Orange killifish
- Source: Niikura fish farm
- Length at study initiation: 2.2 cm (2.0 - 2.3 cm), n=10
- Weight at study initiation: 0.16 g (0.11 - 0.23 g), n=10
- Feeding during test
- Food type: TetraMin
- Amount: 2%/fish weight
- Frequency: daily
ACCLIMATION
- Acclimation period: 54 days
- Acclimation conditions: Same as test
- Type and amount of food: TetraMin - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Test temperature:
- 23.7 - 24.0℃
- pH:
- 7.4 - 7.9
- Dissolved oxygen:
- 5.5 - 8.2 mg/L
- Nominal and measured concentrations:
- nominal concentration: 29, 53, 102 mg/L (and control)
mean measured concentration: 28, 53, 96 mg/L (and control) - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material: glass beaker
- Size: 5.0 L
- Aeration: No
- Type of flow-through: proportional pump
- Renewal rate of test solution: 24.45 mL/min
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Hardness of dilution water: 50 mg/L (as CaCO3)
- pH of dilution water: 7.9
OTHER TEST CONDITIONS
- Photoperiod: 16h light/ 8h dark
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: acute toxicity study (96h); 100 mg/L
- Results used to determine the conditions for the definitive study: 96h-LC50 > 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- Copper(Ⅱ) sulfate pentahydrate
- Duration:
- 7 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 102 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 102 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 102 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 102 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: mortality, toxicological symptoms, body weight, body length,
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 102 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: mortality, toxicological symptoms, body weight, body length,
- Details on results:
- - Observations on body length and weight: No difference
- Mortality of control: 0 - Results with reference substance (positive control):
- - 96h LC50: 0.88 mg/L
- Reported statistics and error estimates:
- Fish weight and length: Dunnett's multiple comparison test, Two-tailed test (α=0.05)
- Executive summary:
In a guideline (OECD 204) and GLP study, MCSI of Japan (1996) reported the 21 -day NOEC for acetonitrile in fish (Oryzias latipes) to be 102 mg/L (nominal conc.) using flow-through exposure conditions.
Reference
1. Method OECD Guidelines for Testing of Chemicals "Fish, Prolonged Toxicity Test: 14 -day Study (Guideline 204, 1984)"
2. Test organisms Oryzias latipes
3. Result 7d LC50>102 mg/L (Nominal concentration) 14d LC50>102 mg/L (Nominal concentration) 21d LC50 >102 mg/L (Nominal concentration) 21d LOEC >102 mg/L (Nominal concentration) 21d NOEC = 102 mg/L (Nominal concentration)
Description of key information
In a guideline (OECD 204) and GLP study, MCSI of Japan (1996) reported the 21-day NOEC for acetonitrile in fish (Oryzias latipes) to be 102 mg/L (nominal conc.) using flow-through exposure conditions.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 102 mg/L
Additional information
In a guideline (OECD 204) and GLP study, MCSI of Japan (1996) reported the 21 -day NOEC for acetonitrile in fish (Oryzias latipes) to be 102 mg/L (nominal conc.) using flow-through exposure conditions.
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