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Long-term toxicity to fish

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Reference
Endpoint:
long-term toxicity to fish, other
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
- Sampling and measured concentration: 0, 7d, 14d, 21d
- Sampling volume: ca. 2 mL
Vehicle:
no
Details on test solutions:
A series of stock solutions (14000, 26000, 50000 mg/L) were prepared by dissolving the test substance in the purified water.

Test organisms (species):
Oryzias latipes
Details on test organisms:
TEST ORGANISM
- Common name: Orange killifish
- Source: Niikura fish farm
- Length at study initiation: 2.2 cm (2.0 - 2.3 cm), n=10
- Weight at study initiation: 0.16 g (0.11 - 0.23 g), n=10
- Feeding during test
- Food type: TetraMin
- Amount: 2%/fish weight
- Frequency: daily


ACCLIMATION
- Acclimation period: 54 days
- Acclimation conditions: Same as test
- Type and amount of food: TetraMin
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Test temperature:
23.7 - 24.0℃

pH:
7.4 - 7.9

Dissolved oxygen:
5.5 - 8.2 mg/L

Nominal and measured concentrations:
nominal concentration: 29, 53, 102 mg/L (and control)
mean measured concentration: 28, 53, 96 mg/L (and control)

Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material: glass beaker
- Size: 5.0 L
- Aeration: No
- Type of flow-through: proportional pump
- Renewal rate of test solution: 24.45 mL/min
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Hardness of dilution water: 50 mg/L (as CaCO3)
- pH of dilution water: 7.9

OTHER TEST CONDITIONS
- Photoperiod: 16h light/ 8h dark

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: acute toxicity study (96h); 100 mg/L
- Results used to determine the conditions for the definitive study: 96h-LC50 > 100 mg/L

Reference substance (positive control):
yes
Remarks:
Copper(Ⅱ) sulfate pentahydrate
Duration:
7 d
Dose descriptor:
LC50
Effect conc.:
> 102 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 102 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
21 d
Dose descriptor:
LC50
Effect conc.:
> 102 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
> 102 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: mortality, toxicological symptoms, body weight, body length,
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
102 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: mortality, toxicological symptoms, body weight, body length,
Details on results:
- Observations on body length and weight: No difference
- Mortality of control: 0
Results with reference substance (positive control):
- 96h LC50: 0.88 mg/L

Reported statistics and error estimates:
Fish weight and length: Dunnett's multiple comparison test, Two-tailed test (α=0.05)

1. Method OECD Guidelines for Testing of Chemicals "Fish, Prolonged Toxicity Test: 14 -day Study (Guideline 204, 1984)"              

2. Test organisms Oryzias latipes

3. Result 7d LC50>102 mg/L (Nominal concentration) 14d LC50>102 mg/L (Nominal concentration) 21d LC50 >102 mg/L (Nominal concentration) 21d LOEC >102 mg/L (Nominal concentration) 21d NOEC = 102 mg/L (Nominal concentration)

Executive summary:

In a guideline (OECD 204) and GLP study, MCSI of Japan (1996) reported the 21 -day NOEC for acetonitrile in fish (Oryzias latipes) to be 102 mg/L (nominal conc.) using flow-through exposure conditions.

Description of key information

In a guideline (OECD 204) and GLP study, MCSI of Japan (1996) reported the 21-day NOEC for acetonitrile in fish (Oryzias latipes) to be 102 mg/L (nominal conc.) using flow-through exposure conditions.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
102 mg/L

Additional information

In a guideline (OECD 204) and GLP study, MCSI of Japan (1996) reported the 21 -day NOEC for acetonitrile in fish (Oryzias latipes) to be 102 mg/L (nominal conc.) using flow-through exposure conditions.