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The following acute toxicity tests with aquatic organisms are available:

Leuciscus idus: LC50 (96 h) > 10000 mg/L; NOEC (96 h) = 10000 mg/L (BASF AG, 2000b)

Daphnia magna: EC50 (48 h) > 100 mg/L; EC0 (48 h) ≥ 100 mg/L (BASF AG, 2000c)

Scenedesmus subspicatus: ErC50 (72 h) > 100 mg/L; ErC10 (72 h) > 100 mg/L; NOEC (72 h) ≥ 100 mg/L (BASF AG, 2000c) Pseudomonas putida: EC50 (30 min) > 10000 mg/L; EC10 (30 min) > 10000 mg/L (BASF AG, 1997).

All values were related to nominal concentrations of suspensions (tests on fish and bacteria) or aqueous extracts (test on daphnids and algae), no analytical monitoring was performed. Previous analytical studies on aqueous extracts of a suspension of this insoluble pigment demonstrated, that the expected concentration of the substance in the extract is below the detection limit of the analytical method. Tests on toxicity to fish indicate that the test substance was suspended in water during the whole exposure period (coloured and cloudy test solution).

However, based on short-term tests from each of the four trophic levels, the substance can be regarded as acutely not harmful to aquatic organisms.

This is in line with the results of a OECD 29 test: The transformation/dissolution concentrations of the most relevant compounds Cr and Sb at a loading rate of 100 mg/L are far below of the acute ERVs and are as well far below the chronic ERVs at a loading rate of 1 mg/L