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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2008
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Good laboratory practice guideline study (OECD).

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008
Report date:
2008

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): (S)-Pentan-2-ol
- Substance type: intermediate
- Physical state: liquid
- Stability under test conditions: pure: stable until 2008-09-30,
in Water: stable under test conditions
- Storage condition of test material: room temperature in the dark
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
not applicable

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:

- Sample storage conditions before analysis: analyzed directly, rest stored in refrigerator (4°C)

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna (Straus), clone 5
- Source: supplied by ECT Ökotoxikologie GmbH, D-65439 Flörsheim am Main
- Age at study initiation: < 24 hours old
- Method of breeding: same water quality as in test (pH, main ions, total hardness)


ACCLIMATION
- Acclimation period: 6 hours
- Acclimation conditions (same as test or not):same as test

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Remarks on exposure duration:
NOT_SPECIFIED
Post exposure observation period:
not applicable

Test conditions

Hardness:
2.5 mmol/l as CaCO3
Test temperature:
19 °C
pH:
7.5 to 7.6
Dissolved oxygen:
at least 8.3 mg/l
Salinity:
freshwater, not applicable
Nominal and measured concentrations:
200 mg/l (nominal) 90 % of nominal (mean, measured)
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass beakers of 250 ml filled with 150 ml.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates):2
- No. of vessels per control (replicates):2


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water
- Alkalinity: 0.8 mmol/l
- Ca/mg ratio: 4:1


OTHER TEST CONDITIONS
- Adjustment of pH: buffered solution

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : EC50 at 24 and 48 h
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
>= 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
none
Results with reference substance (positive control):
not given

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Substance is not toxic or harmful to daphnids.
Executive summary:

The substance was administered to Daphnia magna in a limit test at a concentration of 200 mg/l (nominal) according to OECD guideline 202. No immobilization occured neither after 24 hours nor after 48 h.