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Long-term toxicity to aquatic invertebrates

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Reference
Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
GLP - Guideline study. In accordance to the ECHA guidance document “Practical guide 6: How to report read-across and categories (March 2010)”, the reliability was changed from RL1 to RL2 to reflect the fact that this study was conducted on a read-across substance.
Qualifier:
according to guideline
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
Deviations:
yes
Remarks:
: offspring were counted every workday and not daily
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Preparation of test solutions started with individually prepared loading rates of 1.0, 10 and 100 mg/L in the pre-test and a Ioading rate of 100 mg/L for the final test. A two-day period of magnetic stirring was foIlowed by an overnight stabilisation period. The obtained solutions generally contained undissolved material in the form of a floating layer and precipitate. The aqueous phase of each solution was carefully siphoned off to test vessels. In addition, a ten-fold dilution of the WAF prepared at 1.0 mg/L was tested in the pre-test.
- Differential loading: yes
- Evidence of undissolved material: The final test solutions ware all clear and colourless.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Waterflea
- Strain/clone: Daphnia magna Straus
- Source: In-house Laboratory culture with a known history
- Feeding during test: yes
- Food type: Chlorella pyrenoidosa suspension,
- Amount: 0.2 mg C/daphnia/d, according to OECD 211
- Frequency: daily

BREEDING:
- Start of each batch: With newborn daphnids, i.a less than 3 days old, by placing them individually in 50 mL M7-medium.

- Breeding conditions: Daphnids originated from a healthy Stock, 2nd to 5th brood, showing no signs of stress such as mortality
>20%, presence of males, ephippia or discotoured animals and there was no delay in the production of the tirst brood.
Three times a week the young are counted and the parental daphnids are transferred to new media.

- Type and amount of food: A suspension of fresh water algae
- Feeding frequency: Daily
Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
21 d
Hardness:
143 - 179 mg/L CaCO3
Test temperature:
18.7 - 19.6 °C
pH:
7.6 - 8.4
Dissolved oxygen:
8.2 - 10 mg/L
Nominal and measured concentrations:
Nominal: control and 100 mg/L (WAF)
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 60 ml vessels (6 x 3.5 cm diameter), all-glass covered with a Perspex pIate with 50 mL fill volume
- Renewal rate of test solution (frequency/flow rate): Three times a week
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 20


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M7 medium according to guideline
- Culture medium different from test medium: No


OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours light
- Light intensity:
intensity at the start: 735-748 lux (10.6-10.7 µE m-2*s-1)
intensity at the end: 686 - 694 lux (9.4 - 9.7 µE m-2*s-1)


EFFECT PARAMETERS MEASURED : mobility, body length, reproduction, survival of offspring



RANGE-FINDING STUDY
- Test concentrations: Water Accommodated Fractions prepared at loading rate of 1.0, 10 and 100 mg/l ware tested. A ten-fold dilution of the WAF prepared at 1.0 mg/l and a blank control.
Reference substance (positive control):
not specified
Duration:
21 d
Dose descriptor:
NOELR
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
WAF
Basis for effect:
reproduction

Ashes (residues) from coal fired power station, siliceous ash without co-combustion-ENERGETIKA did not affect reproduction or survival of Daphnia magna at a WAF prepared at a loading rate of 100 mg/L after 21 days of exposure.

Description of key information

No effects were observed up  to a loading rate of 100 mg/L. It can be concluded that cenospheres showes no chronic effects to aquatic invertebrates.

Key value for chemical safety assessment

Additional information

The long term effects of the test substance to aquatic invertebrates were evaluated in a 21d tests withDaphnia magnaaccording to the OECD 211, with "siliceous ash. " The test design was semi-static with a renewal of the test substance and growth medium. The tested ash was structural related to ash (residues), cenospheres and as cenospheres are a component of fly ash, these results can be used as read across. The test substance did not affect reproduction or survival of Daphnia magna at a WAF prepared at a loading rate of 100 mg/L after 21 days of exposure.