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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1981
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Experimental investigation, but study well documented, meeting generally accepted scientific principles, acceptable for assessment.
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
publication
Title:
Sensory irritation caused by various industrial airborne chemicals.
Author:
De Ceaurriz, J.C.; Micillino, J. ; Bonnet, P. ; Guenier, J.
Year:
1981
Bibliographic source:
Toxicol. Lett. 9:137-144.

Materials and methods

Principles of method if other than guideline:
This study was carried out to investigate the sensory irritation potential of a series of airborne chemicals including ethylbenzene. Relative potency was evaluated by determining the RD50 (concentration associated with a 50% reduction in reflwx respiratory rate).
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Ethylbenzene
EC Number:
202-849-4
EC Name:
Ethylbenzene
Cas Number:
100-41-4
Molecular formula:
C8H10
IUPAC Name:
ethylbenzene
Details on test material:
- Name of test material (as cited in study report): Ethylbenzene

Test animals

Species:
mouse
Strain:
other: Swiss OF1
Sex:
male
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: IFFA CREDO Laboratories (France)
- Weight at study initiation: 25 ± 2 g

Administration / exposure

Route of administration:
inhalation
Type of inhalation exposure:
not specified
Vehicle:
other: no data
Details on inhalation exposure:
Groups of 6 male Swiss OF1 mice (weighing 25 g +-2) were used at each of at least 4 different concentrations for each dose level for a period of 5 minutes. The animals were exposed head only enclosed in whole body plethysmographs. A control reading was obtained prior to exposure. The air concentrations of ethylbenzene were monitored by gas chromatography. During exposure the respiratory rate decreased and the maximum % decrease from control values was calculated.
The dose response relationship was obtained by plotting the maximum decrease in respiratory rate against log conc. The dose response curves were obtained by using a least squares linear regression analysis. Each curve showed a linear relationship between log conc and decrease in respiratory rate wich was used to calculate the RD50.
Concentrations:
no data
No. of animals per sex per dose:
no data
Control animals:
not specified
Details on study design:
no data
Statistics:
no data

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
other: RD50
Effect level:
1 432 ppm
Mortality:
no data
Clinical signs:
other: no data
Body weight:
no data
Gross pathology:
no data
Other findings:
no data

Any other information on results incl. tables

None              

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The RD50 for ethylbenzene was found to be 1432 ppm (6.2 mg/l assuming a conversion factor for EB of 1 ppm = 0.00434 mg/l).
Executive summary:

This study was carried out to investigate the sensory irritation potential of a series of airborne chemicals including ethylbenzene. Relative potency was evaluated by determining the RD50 (concentration associated with a 50% reduction in reflwx respiratory rate).

Groups of 6 male Swiss OF1 mice (weighing 25 g +-2) were used at each of at least 4 different concentrations for each dose level for a period of 5 minutes. The animals were exposed head only enclosed in whole body plethysmographs. A control reading was obtained prior to exposure. The air concentrations of ethylbenzene were monitored by gas chromatography. During exposure the respiratory rate decreased and the maximum % decrease from control values was calculated. The dose response relationship was obtained by plotting the maximum decrease in respiratory rate against log conc. The dose response curves were obtained by using a least squares linear regression analysis. Each curve showed a linear relationship between log conc and decrease in respiratory rate wich was used to calculate the RD50.

The RD50 for ethylbenzene was found to be 1432 ppm (6.2 mg/l assuming a conversion factor for EB of 1 ppm = 0.00434 mg/l).