Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 931-257-5 | CAS number: -
Direct MTT reduction
Before the test itselfdirect MTT reduction was assayed.
After 60 min incubation of the test substance with MTT medium, colour was compared with control solution of MTT medium only. Due to presence of dark suspended test substance colour was not clearly appreciable. After filtration no changes in colouring were observed.
The test substance did not reduce MTT directly.
OD570 measuring was performed after overnight extraction.
EVALUATION OF RESULTS
The average viability of affected tissues was 94.6 % of negative control average value after 3 min treatment and 103.2 % after 60 min. These values are higher than critical values (50 and 15% respectively). The substance could be regarded as non-corrosive.
Test substance Fluidized Bed Combustion (FBC) Bottom Ash was assayed for the in vitro skin corrosion in human epidermal model EpiDermTM. The test was performed according to Method B.40. Skin corrosion (in vitro),Council Regulation (EC) No. 440/2008. Published in O.J. L 142, 2008.
The test substance (25 mg) was grinded in mortar with pestle andplaced atop the tissue previously moistened with 25 μl of sterile water. Length of exposition was 3 and 60 minutes. Nine tissues were used for the experiment in each time, three per test substance (TS), three for positive control (PC) and three for negative control (NC).
After rinsing, tissues were incubated with MTT for three hours and extracted overnight subsequently at room temperature without shaking.OD570 of isopropanol extracts was measured on a spectrophotometer. Relative cell viability was calculated for each tissue as % of the mean viability of the negative control tissues.
In the experiment arrangement given above, the test substance Fluidized Bed Combustion (FBC) Bottom Ash was not corrosive in EpiDermTMmodel.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Close Do not show this message again