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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Eye irritation

Currently viewing:

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Older study: meets scientific standards with acceptable restrictions (limited number of animals in study, partly limited documentation).

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1972

Materials and methods

Test guideline
Qualifier:
no guideline followed
Guideline:
other: Study from 1972 (no guidelines available at the time the study was performed)
Deviations:
not applicable
Principles of method if other than guideline:
0.1 mL of undiluted sample was placed in the conjunctival sac of the right eye of each of 3 albino male and female rabbits and observations made over a period of 7 days for inflammation. The eyes were rinsed after 24 hrs and the left eye served as control.
Scoring according to Draize et al. (1944) J Pharm Exp Therap 82
GLP compliance:
no
Remarks:
Study from 1972 (GLP was not compulsory at the time the study was performed)

Test material

Constituent 1
Chemical structure
Reference substance name:
2-phenylpropene
EC Number:
202-705-0
EC Name:
2-phenylpropene
Cas Number:
98-83-9
Molecular formula:
C9H10
IUPAC Name:
(prop-1-en-2-yl)benzene
Details on test material:
Lot/batch No.: 68 T 1
crude test substance (no further data)

Test animals / tissue source

Species:
rabbit
Strain:
other: albino

Test system

Vehicle:
unchanged (no vehicle)
Controls:
not specified
Amount / concentration applied:
0.1 mL
Duration of treatment / exposure:
24 hrs
Observation period (in vivo):
168 hrs
Number of animals or in vitro replicates:
3 male and female NZW rabbits

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 1 hr
Score:
14
Max. score:
110
Reversibility:
fully reversible within: 120 hrs

Any other information on results incl. tables

Numerical evaluations at the end of ... hrs

Animal / Sex

1

24

48

72

120

168

1 - female

14

10

4

2

0

0

2 - female

14

8

4

2

0

0

3 - female

14

8

4

2

0

0

Average

14

8.6

4

2

0

0

Observations following application:

Immediate

Severe discomfort, with pawing, squealing, and trashing about the stocks

10 min

severe erythema, slight oedema, copious discharge

1 hr

moderate erythema, slight oedema, copious discharge

24 hrs

moderate erythema, very slight oedema, slight discharge

48 hrs

slight erythema and discharge

72 hrs

slight erythema

120 hrs

eyes normal; zero readings

168 hrs

eyes normal; zero readings

Applicant's summary and conclusion

Interpretation of results:
slightly irritating
Remarks:
Migrated information
Conclusions:
The substance was slighty irritating.
Executive summary:

In this older study 0.1 mL of undiluted sample was placed in the conjunctival sac of the right eye of each of 3 albino male and female rabbits and observations made over a period of 7 days for inflammation. The eyes were rinsed after 24 hrs and the left eye served as control. Scoring according to Draize et al. (1944) J Pharm Exp Therap 82.

With an average maximum score of 14 in one hour (possible: 110) the TS was classed as slightly irritating in male and female rabbits.