2,3-epoxypropyltrimethylammonium chloride

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 1996-07-30

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The test was carried out in accordance with an appropriate test method, and in compliance with GLP. No substance-specific analysis was performed.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EPA OTS 797.1300 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,3-epoxypropyltrimethylammonium chloride

EC Number:

221-221-0

EC Name:

2,3-epoxypropyltrimethylammonium chloride

Cas Number:

3033-77-0

Molecular formula:

C6H14NO.Cl

IUPAC Name:

N,N,N-trimethyl(oxiran-2-yl)methanaminium chloride

Details on test material:

- Name of test material (as cited in study report): QUAT188

- Physical state: clear liquid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION

- Method: test solutions were prepared by serial dilution of the stock solution

- Controls: control vessels contained 61 mg/L NaCl

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM

- Source: in house

- Age at study initiation (mean and range, SD): <24 h

- Feeding during test: not reported


ACCLIMATION

- Acclimation period: in house breeding

- Acclimation conditions (same as test or not): culture conditions were the same as test conditions.

- Type and amount of food: Ankistrodesmus convolutus and Nitzschia frustulum

- Feeding frequency: 4 times per week

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

166 mg/L as CaCO3

Test temperature:

Temperature ranged from 19.8-20.9 °C

pH:

pH ranged from 7.0-7.8 

Dissolved oxygen:

dissolved oxygen ranged from 8.2-8.7 mg/l i.e. >92.1% saturation

Nominal and measured concentrations:

Nominal concentrations: control (61 mg/L NaCl), 6.3, 12.5, 25, 50 and 100 mg QUAT 188 epoxide/L

Details on test conditions:

TEST SYSTEM

- Test vessel: cups

- Type (delete if not applicable): loosely covered

- Material, size, headspace, fill volume: 250 ml plastic cups filled with 200 ml test solution volume

- No. of organisms per vessel: 10

- No. of vessels per concentration (replicates): 2

- No. of vessels per control (replicates): 2


TEST MEDIUM / WATER PARAMETERS

- Source/preparation of dilution water: Lake Huron water, obtained from upper Saginaw Bay, filtered, autoclaved and pH adjusted.

- Alkalinity: 41 mg/L as CaCO3

- Culture medium different from test medium:

- Intervals of water quality measurement: Dissolved oxygen, pH and temperature were recorded in each test vessel daily.

OTHER TEST CONDITIONS

- Photoperiod: 16h light, 8 h dark cycle

- Light intensity: 1878 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility, every 24 h

TEST CONCENTRATIONS

- Spacing factor for test concentrations: 2 (QUAT188)

- Range finding study

- Test concentrations: 0, 100 and 1000 mg/L

- Results used to determine the conditions for the definitive study: 100% mortality at 1000 mg/L (QUAT 188), 20% mortality at 1000 mg/L (QUAT 188 diol)

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

6.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: immobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

17.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: immobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

12.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: affected

Reported statistics and error estimates:

A computer program with probit analysis, moving average angle analysis and binomial probability/non-linear interpolation  was used to calculate the LC50/EC50 and corresponding 95 % confidence intervals. The order of preference used to select the method for reporting LC50/EC50 was: probit analysis, moving average and binomial/non-linear interpolation.  The computer program attempted to process the data using each of the three methods. However, the appropriateness of a given method was determined in the program by the concentration-response data e.g. the number of concentrations resulting in mortality between 0 and 100 %.

Any other information on results incl. tables

Table 1. Summary of findings.

 

Nominal concentration (mg/L)	# Dead		# Affected
	24 h	48 h	24 h	48 h
0 (water control)	1*	1*	10	10
	0	0	0	0
61 (NaCl control)	0	0	0	0
	0	0	0	0
6.3	0	0*	0	10
	0	0*	0	6
12.5	0	1	0	1
	0	1	0	1
25.0	0	9*	0	10
	0*	10	2	10
50.0	4*	10	6	10
	4*	10	6	10
100.0	10	10	10	10
	10	10	10	10

 

* Immobility noted in surviving organisms

 

 

The results reported as LC50 based on mortality should in fact be expressed as EC50, since it is difficult to determine the definite mortality of small organisms such as D. magna with the naked eye. Therefore in the conclusions the LC50 will be used as EC50.

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

A reliable 48 h EC50 value of 17.2 mg/L has been determined for the effects of QUAT 188 epoxide on the immobility of Daphnia magna.