Registration Dossier

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
no data
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Remarks:
Well documented, scientifically sound study in which 56 rats were administered the test substance intraperitoneally to determine the LD50. Individual animal mortality, clinical signs, body weights, and pathology were not provided. Number of doses evaluated was not given. Sex of the animals was not stated.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1950

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
56 rats were administered the test substance intraperitoneally to determine the LD50.
GLP compliance:
no
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Zirconium dichloride oxide
EC Number:
231-717-9
EC Name:
Zirconium dichloride oxide
Cas Number:
7699-43-6
Molecular formula:
Cl2OZr
IUPAC Name:
Dichloro(oxo)zirconium
Test material form:
solid: crystalline
Details on test material:
- Name of test material (as cited in study report): zirconium oxychloride
- Physical state: crystals

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
not specified
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Age at study initiation: Adults
- Weight at study initiation: 200 - 300 g
- Diet (e.g. ad libitum): ad libitum, purina chow
- Water (e.g. ad libitum): ad libitum

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
water
Details on exposure:
VEHICLE
- Concentration in vehicle: 50%
Doses:
A 50% concentration was used, but the specific number of doses used in the study was not provided
No. of animals per sex per dose:
56 total animals were used (sex of the animals was not provided)
Control animals:
no
Details on study design:
- Duration of observation period following administration: 10 days, an initial group of animals receiving the test substance was kept for 30 days to verify if any significant mortality occurred after the tenth day.
Statistics:
The LD50 values were obtained from 10-day mortaility data by using the log-probability method.

Results and discussion

Effect levels
Sex:
not specified
Dose descriptor:
LD50
Effect level:
ca. 400 mg/kg bw
Based on:
test mat.
Mortality:
The LD50 was calculated to be 400 mg compound/kg (113 mg metal/kg). Individual animal mortality data was not provided.
Clinical signs:
Animals exposed to the test substance showed a progressive depression and decrease in activity until death occurred. The time of death varied from a few hours to a few days following the exposure to the test substance. Few deaths were reported later than five days after exposure to test substance.
Body weight:
no data
Gross pathology:
No gross pathological changes were reported in any of the animals receiving lethal doses of the test substance.
Other findings:
No physiological changes were reported in any of the animals receiving lethal doses of the test substance.

Applicant's summary and conclusion

Conclusions:
The LD50 was calculated to be 400 mg/kg bw after intraperitoneal administration.