2-chlorobuta-1,3-diene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: acceptable well-documented publication which meets basic scientific principles

Data source

Reference

Reference Type:

publication

Title:

Toxicity of chloroprene in an acute test during oral administration

Author:

Asmamgulian, T., A.; et al.

Year:

1971

Bibliographic source:

Proceedings of the Yerevan Medical Institute, vol. XV, 461-465

Materials and methods

Principles of method if other than guideline:

other: acute oral toxicity study

GLP compliance:

no

Test type:

other: acute oral toxicity study

Test material

Test material information

Constituent 1

Reference substance name:

2-chlorobuta-1,3-diene

EC Number:

204-818-0

EC Name:

2-chlorobuta-1,3-diene

Cas Number:

126-99-8

Molecular formula:

C4H5Cl

IUPAC Name:

2-chlorobuta-1,3-diene

Details on test material:

chloroprene

Test animals

Species:

rat

Strain:

not specified

Sex:

not specified

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: sunflower seed oil

Doses:

150 to 400 mg/kg bw

No. of animals per sex per dose:

total number of rats used: 54

Control animals:

yes

Results and discussion

Effect levels

Sex:

not specified

Dose descriptor:

LD50

Effect level:

251 mg/kg bw

Based on:

test mat.

Remarks on result:

other: clinical signs, death, pathological changes in the inner organs and brain membrane

Any other information on results incl. tables

Clinical signs: symptoms of a depressed nervous system, animals were apathetic, death occured usually within the first day, survivors were comparable to the control at study termination

Autopsy of decedent: irregular blood content of the inner organs, hemorrhages foci in the brain membrane

Microscopic evaluations of decedents: blood vessels of the liver plethoric showed a turbid swelling, some lung alveoles were collapsed, lung tissue with formolic pigment, diapedesis of erythrocytes, epicardium was loosened up in spots, muscle fibers showed a turbid swelling, the fatty tissue of myocardium under epicardium and endocardium with foci of hemorrhages, the pulp of the spleen was plethoric with aggregated of eosinophiles, epithelium of the convoluted channels in the kidneys with distinct granular dystrophy, vessel of the brain cortex showed plethoric, the brain vessels were severely plethoric and had foci of hemorrhages, the brain tissue was swollen, stomach with a moderate leuco- and lymphocytic infiltration and a pronounced accumulation of eosinophiles

Applicant's summary and conclusion

Executive summary:

Oral LD50 value in rats: 251 mg/kg bw; the clinical picture of acute poisoning is characterized by depression of the central nervous system. Patho-morphological changes in decedents after single oral application were mainly plethora, hemorrhages and dystropic changes in the central nervous system and in the inner organs.