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EC number: 266-946-3 | CAS number: 67701-28-4 This substance is identified by SDA Substance Name: C8-C18 and C18 unsaturated trialkyl glyceride and SDA Reporting Number: 01-001-00.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- weight of evidence
- Study period:
- From Apr. 11, 1988 to Apr. 15, 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to OECD Guideline 203. No analytical verification of test concentrations.
- Justification for data waiving:
- other:
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- not specified
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes
Test material
- Reference substance name:
- Glycerides, C16 and C18-unsatd. and C18-unsatd. hydroxy
- IUPAC Name:
- Glycerides, C16 and C18-unsatd. and C18-unsatd. hydroxy
- Details on test material:
- - Name of test material (as cited in study report): Castor oil (CAS N° 8001-79-4, EC N° 232-293-8); under the SDA nomenclature, the name of this substance is ‘Glycerides, C16 and C18-unsatd. and C18-unsatd. hydroxy’
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- No data
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Stock solutions of the test material were prepared by mechanical dissolving (Ultra-Turrax) the appropriate amount of the test material in the required volume of deionised water. This stock solution was transferred into the test medium of one glass tank. Loading of the test medium with the test sample was done 2 h before the start of the test. Until the transfer of fish into the test solution, the contents of each glass tank were mixed thoroughly using a mechanical stirrer in order to facilitate the dispersing of the test sample.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra-fish
- Strain: Brachydanio rerio (Teleostei, Cyprinidae)
- Source: West Aquarium GmbH + Co. KG, D- 3422 Bad Lauterberg
- Age at study initiation (mean and range, SD): Two months (approx.)
- Length at study initiation (length definition, mean, range and SD): 2.0 ± 1.0 cm
ACCLIMATION
- Acclimation period: 13 d
- Acclimation conditions: The fish was transferred to a tank with a total water volume of about 200 L for adaptation to the environmental conditions during the test. During the adaptation period, the holding of the test fish was performed using a semi-static system in which the reconstituted water was recirculated and filtered continuously.
- Type and amount of food: Tetra Min Hauptfutter (Art.-No. 1004 DN, produced by Tetra-Werke, Dr. rer.nat. Ulrich Baensch GmbH, D- 4520 Melle 1)
- Feeding frequency: Twice daily
- Health during acclimation (any mortality observed): Good health, no mortality observed
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- None
Test conditions
- Hardness:
- No data
- Test temperature:
- 20.8-21.8
- pH:
- 7.5-7.6
- Dissolved oxygen:
- >86% (at 96 h)
- Salinity:
- No data
- Nominal and measured concentrations:
- 100 and 1000 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: The glass tanks for the different test batches had the following dimensions: length 300 mm, width 200 mm and height 220 mm.
- Aeration: For the continuous aeration of the test medium in the different glass tanks special aquarium aerators, brand Optima (Art.-No. A 10007, produced by Weltweit GmbH & Co. KG, D- 2081 Holm near Hamburg) were used. Air was bubbled into the test medium through Pasteur pipettes directly connected with the aerator by flexible PVC tubes. The air-stream was regulated with tube-clamps providing for each tank a uniform air-stream of 1 to 3 bubbles per second.
- No. of organisms per vessel: 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of reconstituted water: It was prepared according to the Annex of OECD-Guideline 203 in the following way (referred to a total volume of 10.0 L):
117.6 g Calcium chloride dihydrate CaCl2.2 H2O (Art.No. 2382, produced by E. Merck AG, D- 6100 Darmstadt) were dissolved in deionised water; this solution was made up with deionised water to a total volume of 1.0 L.
49·3 g Magnesium sulfate heptahydrate MgSO 4.7 H2O (Art.-No. 5886 produced by E. Merck AG, D-6100 Darmstadt) were dissolved in deionised water; this solution was made up with deionisd water to a total volume of 1.0 L.
25.9 g Sodium hydrogencarbonate O 3 (Art.-No. 6329, produced by E. Merck AG, D-6100 Darmstadt) were dissolved in deionised water; this solution was made up with deionised water to a total volume of 1.0 L.
2.3 g Potassium chloride KCl (Art.-Noo 4936, produced by E. Merck AG, D-6100 Darmstadt) were dissolved in deionised water; this solution was made up with deionised water to a total volume of 1.0 L.
For the preparation of the reconstituted water, 25 mL each of the four afore-mentioned solutions were mixed; this mixture was then made up with deionised water to a total volume of 10.0 L Thus reconstituted water prepared in the described way, met the following requirements:
The sum of the calcium and magnesium ions was 2.5 mmol/L. The molar ratio of calcium to magnesium ions was 4:1. The molar ratio of sodium to potassium ions was 10:1. The total alkalinity or acid capacity was 0.8 mmol/L.
- Conductivity: < 0.1 µS/cm
OTHER TEST CONDITIONS
- Adjustment of pH: Yes, determination of pH was carried out with the measuring device pH 91/Set (Art.-No. 9.774091, produced by WTW, D-8120 Weilheim)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality rates, abnormal symptoms in the swimming behaviour, in visible physiological functions as well as in the outward appearance of the test fish were observed at 0, 24, 48, 72 and 96 h.
- Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Charging the test medium with the test substance led to a flocky dispersion slowly settling up in the course of the test period.
- Results with reference substance (positive control):
- No data
- Reported statistics and error estimates:
- No data
Any other information on results incl. tables
- Sublethal observations / clinical signs:
None
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Under the test conditions, the 96 h LC0 of castor oil for (Brachydanio rerio) was found to be 1,000 mg/L (based on nominal concentrations).
- Executive summary:
A study was conducted to determine the lethal effects of castor oil to the Zebra-fish (Brachydanio rerio) using a static system according to the OECD Guideline 203.
Fish were exposed in separate test batches to different concentration (100 and 1000 mg/L) of the test material in the test medium over a test period of 96 h (each tank with 10 fish). Observations were made at 0, 24, 48, 72 and 96 h for the mortality rates, abnormal symptoms in the swimming behaviour, in visible physiological functions as well as in the outward appearance of the test fish. Fish were considered dead if touching of the caudal peduncle produced no reaction and if no breathing movements could be observed.
Under the test conditions, the 96 h LC0 for (Brachydanio rerio) was found to be 1,000 mg/L (based on nominal concentrations).
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