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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Reference
Endpoint:
toxicity to soil macroorganisms except arthropods: long-term
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 222 (Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei))
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Vehicle:
yes
Details on preparation and application of test substrate:
The stock solution was prepared in ethanol. An appropriate amount of the stock and the dosage solutions, respectively was used to soak a
portion of the quartz sand of the artificial soil. After evaporation of the solvent by placing under a fume hood, the quartz sand was incorporated into the remainders of the artificial soil to prepare the different test item soil concentrations.
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
TEST ORGANISM
- Common name: Earthworms
- Source: ECT Oekotoxikologie GmbH since February 1994; originally delivered by Co. Landenberger (72355 Schömberg, Germany)
- Age at test initiation (mean and range, SD): 2 - 12 months
- Weight at test initiation (mean and range, SD): 300 - 600 mg

ACCLIMATION
- Acclimation period: 2 days
- Acclimation conditions (same as test or not): same as test
- Health during acclimation (any mortality observed): healthy
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
56 d
Test temperature:
18.3 – 21.6 °C
pH:
20 ± 2 ºC
Moisture:
50.7 - 59.9 % of WHCmax
Nominal and measured concentrations:
Concentrations tested: Solvent control, control; 18.0, 32.0, 56.0, 100, 180 and 320 mg test item/kg artificial soil (dw).

Measured 95.3 - 97.7 % of nominal in the test solution of the highest concentration.
Reference substance (positive control):
yes
Remarks:
Carbendazim
Duration:
56 d
Dose descriptor:
NOEC
Effect conc.:
< 18 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
56 d
Dose descriptor:
EC10
Effect conc.:
11.2 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction

Description of key information

An experimental study with Eisenia fetida predicted an EC10 of 11.2 mg/kg soil dw, after 56 d exposure to the test item

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for soil macroorganisms:
11.2 mg/kg soil dw

Additional information

An experimental study with Eisenia fetida according to OECD guideline 222 and GLP regulations assessed the long-term toxicity of the test item to soil macroorganisms. An EC10 of 11.2 mg/kg soil dw was obtained after 56 d exposure time based on the reproduction of the test species (ECT, 2012). A supporting study with the same species but a short exposure period and a higher maximal test concentration revealed a NOEC of 180 mg/kg soil dw and an EC50 of 444 mg/kg soil dw (TNO, 1992). Since these results are based only on nominal concentrations and no analytical monitoring was conducted, they are only used as supporting data which give a hint for a possible lower toxicity of the test item to soil macroogranism than predicted by the ECT study. Assuming the worst-case further assessments are made based on the ECT results.