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EC number: 239-268-0 | CAS number: 15214-89-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well documented report of a guideline study conducted according to GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 475 (Mammalian Bone Marrow Chromosome Aberration Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- chromosome aberration assay
Test material
- Reference substance name:
- 2-acrylamido-2-methylpropanesulphonic acid
- EC Number:
- 239-268-0
- EC Name:
- 2-acrylamido-2-methylpropanesulphonic acid
- Cas Number:
- 15214-89-8
- Molecular formula:
- C7H13NO4S
- IUPAC Name:
- (1Z)-N-(2-methyl-1-sulfopropan-2-yl)prop-2-enimidic acid
- Details on test material:
- - Name of test material (as cited in study report): OS 61349J (CASRN = 15214-89-8)
- Substance type: organic
- Physical state: solid
- Analytical purity: not specified
- Impurities (identity and concentrations): not specified
- Composition of test material, percentage of components: 100%
- Purity test date: not specified
- Lot/batch No.: not specified
- Expiration date of the lot/batch: not specified
- Stability under test conditions: stable
- Storage condition of test material: room temerature, protected from light
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 8-9 weeks old
- Fasting period before study: no
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- - Vehicle used: water
- Frequency of treatment:
- single dose
- Post exposure period:
- 6, 18 and 24 hours
Doses / concentrations
- Remarks:
- Doses / Concentrations:
150; 500 and 1500 mg/kg
Basis:
actual ingested
- No. of animals per sex per dose:
- 5
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- cyclophosphamide
- Route of administration: gavage
- Doses / concentrations: 30 mg/kg
Examinations
- Tissues and cell types examined:
- Bone marrow cells
- Details of tissue and slide preparation:
- Bone marrow cells were centrifuged and resuspended in fresh hypotonic solution and incubated for 20 minutes at 37°C in a water bath. Following hypotonic treatment, cells were centrifuged and resuspended in fixative solution of 3 parts methanol and 1 part acetic acid. Cells were treated with fresh fixative solution 4 times by centrifugation and resuspension. Drops of the concentrated cell suspension were placed on clean moist glass slides. Slides were dried at least 24 hours and stained with 5% Gurr R66 Giesma for approximately 5-6 minutes at room temperature. The cell harvest, slide preparation and staing techniques followed standard cytogenic procedures.
- Statistics:
- The percentage of cells with chromosomal aberration were analyzed by the statistical methods described by Margolin et al., Environmental Mutagenesis, Volume 8, 1986.
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- yes
- Vehicle controls validity:
- valid
- Negative controls validity:
- not examined
- Positive controls validity:
- valid
Any other information on results incl. tables
Clinical signs observed in the animals dosed at 1500 mg/kg included increased respiratory rate (50%), watery feces (28%), soft feces (17%), and wheezing (1%). Mean body weights of the dose groups were not significantly different from the vehicle control group at any time point. As is illustrated in Table 1 below, the test article did not produce statistically significant increases (p<0.05) in the percentage of cells with aberrations at any dose, time period or for either sex, compared to control values. Positive control animals treated with cyclophosphamide (30 mg/kg) demonstrated an increase in the frequency of damaged cells which was statistically significant at the study threshold of p<0.05. Thus, the positive and negative controls demonstrated the reliability of the assay to detect chromosomal aberrations.
Table 1:
Male | Female | ||||||
Time (hours) |
Treatment | Dose (mg/kg) |
% cells with aberrations |
Time (houirs) |
Treatment | Dose (mg/kg) |
% cells with aberrations |
6 | Vehicle | 3.2 | 6 | Vehicle | 2.0 | ||
OS61349J | 150 | 0.8 | OS61349J | 150 | 2.0 | ||
500 | 1.6 | 500 | 0.8 | ||||
1500 | 1.2 | 1500 | 1.2 | ||||
18 | Vehicle | 0.4 | 18 | Vehicle | 0.4 | ||
OS61349J | 150 | 0.4 | OS61349J | 150 | 1.2 | ||
500 | 0.4 | 500 | 0.8 | ||||
1500 | 0.8 | 1500 | 1.2 | ||||
24 | Cyclophosphamide | 30 | 18.8 | 24 | Cyclophosphamide | 30 | 19.2 |
Vehicle | 0.8 | Vehicle | 1.6 | ||||
OS61349J | 150 | 0.0 | OS61349J | 150 | 1.2 | ||
500 | 0.8 | 500 | 2.4 | ||||
1500 | 0.8 | 1500 | 0.8 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
The test material was non-clastogenic in rat bone marrow cells under the conditions of the assay.
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