Registration Dossier
Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 239-268-0 | CAS number: 15214-89-8
Ecotoxicological information
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1983
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well documented report of a guideline study conducted according to GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: EPA-66013-75-009, "methods for acute toxicity tests with fish, macrovertebrates, and amphibians.
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-acrylamido-2-methylpropanesulphonic acid
- EC Number:
- 239-268-0
- EC Name:
- 2-acrylamido-2-methylpropanesulphonic acid
- Cas Number:
- 15214-89-8
- Molecular formula:
- C7H13NO4S
- IUPAC Name:
- (1Z)-N-(2-methyl-1-sulfopropan-2-yl)prop-2-enimidic acid
- Details on test material:
- - Name of test material (as cited in study report): OS #26935F
- Substance type: organic
- Physical state: solid
- Analytical purity: not specified
- Impurities (identity and concentrations): not specified
- Composition of test material, percentage of components: 100%
- Purity test date: not specified
- Lot/batch No.: not specified
- Expiration date of the lot/batch: not specified
- Stability under test conditions: stable
- Storage condition of test material: not specified
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: dilution
- Chemical name of vehicle: soft water reconstituted from deionized water
- stock solution: 150 mg/mL
- final test solutions: 130, 220, 360, 600 and 1000 mg/L
Test organisms
- Test organisms (species):
- Lepomis macrochirus
- Details on test organisms:
- TEST ORGANISM
- Common name: Bluegill
- Source: in house laboratory supply (Lot # 83A13)
- Age at study initiation: juvenile
- Length at study initiation: 3.8 cm
- Weight at study initiation: 0.66 g (wet weight)
- Feeding during test: no feeding during the study
ACCLIMATION
- Acclimation period: 14 days prior to start the test
- Acclimation conditions: same as test
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 44 mg/L as CaCO3
- Test temperature:
- 22°C +/- 1
- pH:
- 7.5
- Dissolved oxygen:
- 5.5 to 9.2 mg 02/L
- Nominal and measured concentrations:
- Nominal concentration:
Control, 130; 220; 360; 600 andd 1000 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: closed
- Material, size, headspace, fill volume: glass exposure vessels containing 15 L of test solution
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): no replicates
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: soft water reconstituted from dionized water
- Alkalinity: 32 mg/L
- Conductivity: 140 microhos/cm
OTHER TEST CONDITIONS
- Photoperiod: 16 hours of light and 8 hours darkness
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.6 - 1.7 - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 130 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 170 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 130 - 220
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 170 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 130 - 220
- Details on results:
- - Abnormal responses: sublethal effects including loss of equilibrium and rapid respiration was seen at 0 hours in the 600 and 1000 mg/L test concentration
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC0 at 96 hours for fish of ATBS was 130 mg/L and the LC50 at both 72 and 96 hours was calculated as 170 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
