Registration Dossier
Registration Dossier
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EC number: 239-268-0 | CAS number: 15214-89-8
Environmental fate & pathways
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1986
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well documented report of a guideline study conducted according to GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: 40 CFR 795.3340
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Sodium 2-methyl-2-[(1-oxoallyl)amino]propanesulphonate
- EC Number:
- 225-948-4
- EC Name:
- Sodium 2-methyl-2-[(1-oxoallyl)amino]propanesulphonate
- Cas Number:
- 5165-97-9
- IUPAC Name:
- sodium 2-(acryloylamino)-2-methylpropane-1-sulfonate
- Details on test material:
- - Name of test material (as cited in study report): OS6134?E
- Molecular formula (if other than submission substance): C7H13NO4S.Na
- Molecular weight (if other than submission substance): 229.23
- Smiles notation (if other than submission substance): [Na+].CC(C)(CS(=O)(=O)[O-])NC(=O)C=C
- InChl (if other than submission substance): InChI=1/C7H13NO4S.Na/c1-4-6(9)8-7(2,3)5-13(10,11)12;/h4H,1,5H2,2-3H3,(H,8,9)(H,10,11,12);/q;+1/p-1
- Substance type: acrylamide
- Physical state: solid
- Analytical purity: not specified
- Impurities (identity and concentrations): not specified
- Composition of test material, percentage of components: 100%
- Purity test date: not specified
- Lot/batch No.: not specified
- Expiration date of the lot/batch: not specified
- Stability under test conditions: stable
- Storage condition of test material: ambient temperature
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, industrial (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge : O'Bannon Creek domestic wastewater treatment plant
- Laboratory culture: raw sewage collected from a primary settling tank was used as the influent during acclimation period and test periods;
- Preparation of inoculum for exposure: At the end of the acclimation period, sludge from all units was pooled and mixed. Adequate amount was added to each unit to achieve a suspended solids concentration of 1500 mg/L for each unit. One mg carbon/100 ml influent was added to the appropriate test units.
- Pretreatment: The acclimation process included aeration for 23 hours, settling of sludge, removing 100 ml of supernatant and resuming aeration to 500 mg/minute. The units were fed raw sewage daily till a clear supernatant liquor was obtained.
Concentration of test chemical: From day 1 to day 8, 20 mg carbon/l was introduced into the test unit. This was reduced to 10 mg carbon/l from day 8 on. No organic solvents were used to facilitate the dispersion of the test material. The test substance was weighed onto a teflon coupon and introduced into the medium. - Duration of test (contact time):
- 44 d
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Solubilising agent: no
- Aeration of dilution water: 500 mg/minute
- Suspended solids concentration: 1500 mg/L for each unit
TEST SYSTEM
- Culturing apparatus: aeration units consisting of glass vessels with cone shaped lower ends. Units contained 150 ml of mixed liquor when filled
SAMPLING
- Sampling frequency: collected all workdays
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
Results and discussion
% Degradation
- Parameter:
- % degradation (DOC removal)
- Value:
- < 10
- Sampling time:
- 44 d
- Details on results:
- Thes study was extended to 44 days to determine if acclimation and biodegradation would increase in this time frame.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: <10% after 44 days
- Conclusions:
- The test substance showed a low biodegradation rate of less than 10% after 44 days. ATBS was not considered to be readily biodegradable.
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