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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Study period:
No data
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
other company data
Title:
Unnamed
Year:
1967
Report date:
1967

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
Version / remarks:
Conducted prior to adoption of OECD test guidelines.
Deviations:
yes
Remarks:
Limited details on methods and animal conditions.
Principles of method if other than guideline:
Method: other: Not stated. Insufficient detail to fully assess comparability with OECD guideline.
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Nitrilotrimethylenetris(phosphonic acid)
EC Number:
229-146-5
EC Name:
Nitrilotrimethylenetris(phosphonic acid)
Cas Number:
6419-19-8
Molecular formula:
C3H12NO9P3
IUPAC Name:
nitrilotrimethylenetris(phosphonic acid)

Test animals

Species:
rabbit
Strain:
New Zealand White
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: No data
- Age at study initiation: No data
- Weight at study initiation: Males: 2.3-2.5 kg. Females: 2.0 and 2.1 kg.
- Fasting period before study: No data
- Housing: Individually (cage type to stated)
- Diet (e.g. ad libitum): No data
- Water (e.g. ad libitum): No data
- Acclimation period: No data


ENVIRONMENTAL CONDITIONS
- Temperature (°C): No data
- Humidity (%): No data
- Air changes (per hr): No data
- Photoperiod (hrs dark / hrs light): No data


IN-LIFE DATES: No data

Administration / exposure

Type of coverage:
occlusive
Vehicle:
water
Details on dermal exposure:
TEST SITE
- Area of exposure: No data
- % coverage: No data
- Type of wrap if used: "plastic strips"


REMOVAL OF TEST SUBSTANCE
- Washing (if done): No data


TEST MATERIAL
- Amount(s) applied (volume or weight with unit): No data
- Concentration (if solution): 25% aqueous solution
- Constant volume or concentration used: yes


VEHICLE
- Amount(s) applied (volume or weight with unit): No data
- Concentration (if solution): 25% solution
Duration of exposure:
24 hours
Doses:
1000, 1580, 2510, 3980 and 6310 mg/kg bw
No. of animals per sex per dose:
One
Control animals:
no
Details on study design:
- Duration of observation period following administration: Not clear, but at least five days.
- Frequency of observations and weighing: Body weights appear to have been measured prior to dosing and five days after dosing.
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs and gross pathology of animals that die.
Statistics:
None

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 6 310 mg/kg bw
Based on:
act. ingr.
Remarks:
active acid
Mortality:
No deaths occurred at any dose.
Clinical signs:
Activity and appetite were temporarily reduced after doses of 3980 and 6310 mg/kg bw.
Body weight:
No apparent effects.
Gross pathology:
No deaths so not conducted.
Other findings:
None

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
In the key acute dermal toxicity study conducted prior to the adoption of OECD test guidelines and GLP, but according to a protocol similar to OECD Test Guideline 402, the LD50 for ATMP-H (aqueous solution containing 25% w/w active acid) was concluded to be >6310 mg active acid/kg bw.

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