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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

Short term toxicity to fish was moderate (96 hr LC50 values: Golden orfe 17.1 mg/L; Fathead minnow 28.2 mg/L)

Key value for chemical safety assessment

LC50 for freshwater fish:
17.1 mg/L

Additional information

Valid data on the short term toxicity of 2-ethylhexanol to fish indicate moderate toxicity:






96 hr LC50


Leuciscus idus melanotus; Golden orfe



96 hr LC50


Pimephales promelas;

Fathead minnow



Two reliable studies (without restrictions) are available resulting in very similar LC50 (96 h) values, both applied flow through design:

The key study from Huels (1991) was performed on Leuciscus idus (Golden orfe) and involved Marlovet R.40 as a vehicle (28 mg/L). No lethality was observed in the control and solvent control. Actual concentrations were determined by analytical monitoring, and the LC50 (96 h) based on mean measured concentrations was determined as 17.1 mg/L. The study was performed according to EU-method C.1 and compliant to GLP.

The second reliable (RL 1) key study (Geiger et al., 1985) used Fathead minnows (Pimephales promelas) as test organisms and no vehicle was applied. Also in this flow-through study, determined LC50 (96 h) of 28.2 mg/L is based on mean measured concentrations and corroborates results from Huels (1991).

Due to the obviously higher sensitivity of Golden orfe, the LC50 (96 h) of 17.1 mg/L is used for chemical safety assessment.

In addition, other, non-reliable acute toxicity studies on fish resulting in similar or higher LC50 -values are in support of the key studies.