Registration Dossier
Registration Dossier
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EC number: 253-575-7 | CAS number: 37640-57-6
Immunotoxicity
Administrative data
- Endpoint:
- immunotoxicity
- Remarks:
- other: mitogenesis test
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Scientific publication with appropriate description of the method.
Data source
Reference
- Reference Type:
- publication
- Title:
- Immunotoxicological Evaluation of Environmental Chemicals Utilizing Mouse Lymphocyte Mitogenesis Test
- Author:
- Sakazaki H, Ueno H, Umetani K, Utsumi H, Nakamuro K
- Year:
- 2 001
- Bibliographic source:
- J. Health Sci., Vol. 47(3), 258-271, 2001
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The aim of this study was to assess the immunotoxicity of environmentally relevant chemicals including melamine by means of an in vitro lymphocyte mitogenesis test. The growth-inhibitory effect of environmental chemicals was determined on the mouse splenic lymphocyte mitogenesis using lipopolysaccharide and concanavalin A as the specific mitogens for B cells and T cells. The toxicity on humoral immunity and cell-mediated immunity was evaluated, respectively.
When lymphocytes are stimulated by a mitogen, the following reactions occur on the lymphocytes: capping of the receptors or immunoglobulins on the surface of lymphocytes, increase of intracellular calcium concentration, activation of intracellular signaling pathways, phosphorylation and activation of each enzyme, synthesis of RNA and proteins, replication of DNA, and finally mitosis of lymphocytes. If lymphocytes have been exposed to an environmental chemical during the period of the mitogenesis, any inhibition in the sequential reactions can be detected as depression of lymphocyte proliferation. Therefore, the lymphocyte mitogenesis test may be useful for assessing immunotoxicity of environmental chemicals. - GLP compliance:
- not specified
Test material
- Reference substance name:
- Melamine
- EC Number:
- 203-615-4
- EC Name:
- Melamine
- Cas Number:
- 108-78-1
- Molecular formula:
- C3H6N6
- IUPAC Name:
- 1,3,5-triazine-2,4,6-triamine
- Details on test material:
- melamine
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- other: C3H/He and BALB/c
- Sex:
- male
Administration / exposure
- Route of administration:
- other: in vitro assay
- Duration of treatment / exposure:
- Exposure period: 4 day(s)
- Control animals:
- yes
- Details on study design:
- Mouse Lymphocyte Mitogenesis Test
Results and discussion
Any other information on results incl. tables
Melamine did not inhibit the mitogenesis of B- and T-lymphocytes.
Applicant's summary and conclusion
- Conclusions:
- Melamine did not inhibit the mitogenesis of B- and T- lymphocytes.
- Executive summary:
Melamine did not inhibit the mitogenesis of B- and T-lymphocytes.
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