1,3,5-triazine-2,4,6(1H,3H,5H)-trione, compound with 1,3,5-triazine-2,4,6-triamine (1:1)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study performed according to guideline, but no data on substance available and no GLP No analytics performed

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

no

Analytical monitoring:

no

Details on sampling:

no data

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The dilutions were prepared with drinking water and were aerated during the testing period
- Controls: yes (without test substance)

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source:Fischzucht Aquafarm Ryba Zeven

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

no data

Test temperature:

24.8-25.0°C

pH:

7.51-8.40

Dissolved oxygen:

7.9-8.2 mg/L

Nominal and measured concentrations:

Nominal concentration: 10000 mg/L

Details on test conditions:

TEST SYSTEM
- Aeration: yes
- No. of organisms per vessel: 7 fish/12 L
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: ≤ 1 g fish/L

TEST MEDIUM / WATER PARAMETERS
- Drinking water

OTHER TEST CONDITIONS
- Photoperiod: 16 h light/ 8 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
The mortality was recorded every 24 h
The pH, temperature and dissolved oxygen were checked every 24 h

TEST CONCENTRATIONS
- Range finding study: 3 concentrations were tested (10000, 1000 and 100 mg/L), and a control was run in parallel.
No mortality was observed with the 96h testing period.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality was observed during the 96h testing period (control and limit test).
The concentration was not analysed because in the ready biodegradability study, it was proven that the stability of the test substance is ≥ 80% within 5 days.

Results with reference substance (positive control):

no data

Validity criteria fulfilled:

yes

Conclusions:

The 96h-LC50 of the test substance is > 10000 mg/L.

Description of key information

 There is a high probability that the product is not acutely harmful to fish.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

10 000 mg/L

Additional information

The key study performed according to OECD test guideline 203 and under consideration of GLP did not reveal toxic effects after 96h up to a concentration of 10000 mg/L. The LC50 was determined to be 10000 mg/L and the NOEC > 10000 mg/L.

Furthermore, several studies on melamine and cyanuric acid themselves are availalbe.

For the component CAS 108 -78 -1 (melamine):

Five studies, with different reliabilities and with 4 different species of fish are available (Goodrich 1984, Vaessen 1982, Pagga 1979, Nite 2010, Jansen 1978). The report of each of the 5 studies point to the same result: the acute toxicity of melamine to fish is low. The LC50s are in the range of >500 to >4600 mg/L.

 

For the component CAS 108 -80 -5 (Cyanuric acid):

The LC50 has been determined to be 8000 mg /L nominal (Anderson 2002).