Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 919-446-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Sept. 6, 1996-Dec. 9, 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all concentrations
- Sample storage conditions before analysis: If samples could not be analysed the day they were taken, they were stored at 4°C and extracted at the earliest opportunity. Samples were stable for up to 7 days. - Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test substance was added to measured volumes of test medium. The vessels were then sealed, leaving a small headspace, and the contents stirred with a 1-2 cm vortex depth until the aqueous and test substance phases equilibrated (45 hrs). The vessels were then allowed to settle for 1-1.5 hrs and the aqueous phase drawn off for use in the test as a Water Accomodated Fraction (WAF). Control media was treated in the same manner. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain: Strauss
- Source: clonal laboratory culture derived from animals obtained from Zeneca Brixham Laboratory
- Age at study initiation (mean and range, SD): less than 24 hrs
- Method of breeding: Cultures started with animals less than 24 hours old, and discarded when 28 days old. Young are removed daily, and animals for testing removed 23 hours after last removal. Test young are from 15-28 day cultures from at least the third brood. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 173 mg/l CaCO3
- Test temperature:
- 19.4-19.7°C
- pH:
- 7.7-7.9
- Dissolved oxygen:
- 8.0-8.7
- Nominal and measured concentrations:
- Nominal loading rate: 0, 1, 2.2, 4.6, 10, 22, 46, 100 mg/l
Mean concentration of hydrocarbons: <0.05, 0.18, 0.83, 0.85, 1.7, 2.8, 4.7, 7.3 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: sealed with a screw cap
- Material, size, headspace, fill volume: 150 ml Erlenmeyer flasks completely filled
- Renewal rate of test solution (frequency/flow rate): static
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reverse osmosis filtered water with the following Analar grade salts added: NaHCO3 192 mg/l, CaSO4.2H2O120 mg/l, MgSO4.7H2O 240 mg/l, KCl 8 mg/l
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilization
TEST CONCENTRATIONS
- Range finding study: Range finding test was conducted similar to the main study except chemical analysis was not done. - Key result
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- 10 - 22 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EL50
- Effect conc.:
- 22 - 46 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- other: NOEL
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: None
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hr EL50 range for an invertebrate, Daphnia magna, was 10-22 mg/L (WAF).
- Executive summary:
The purpose of this experiment was to determine the acute toxicity of low aromatic white spirits to Daphnia magna. 10 daphnia per test vessel (2 test vessels per concentration) were exposed to loading rates of 0, 1, 2.2, 4.6, 10, 22, 46, or 100 mg/l (WAF) of test substance. The exposure lasted for 48 hrs, and there was no renewal of test solution. At 24 and 48 hrs, the Daphnia were observed for immobilization. Immobilization was seen at test concentrations of 10 mg/l (WAF) and above, with immobilization of all daphnids at the 46 and 100 mg/l concentrations by the 24 hr observation. The 48-hr EL50 range for an invertebrate, Daphnia magna, was 10-22 mg/L (WAF).
Reference
Number of Immobilized Daphnia magna
Loading Rate (mg/l) |
24 hrs |
48 hrs |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
1 |
0 |
0 |
2.2 |
0 |
0 |
2.2 |
0 |
0 |
4.6 |
0 |
0 |
4.6 |
0 |
0 |
10 |
0 |
0 |
10 |
0 |
1 |
22 |
3 |
6 |
22 |
1 |
8 |
46 |
10 |
10 |
46 |
10 |
10 |
100 |
10 |
10 |
100 |
10 |
10 |
Description of key information
There is data available for this substance. Key information is summarized below.
Hydrocarbons C9-C12, n-alkanes, isoalkanes, cyclics, aromatics (2-25%) presented a 48-h EL50 (mobility) range for Daphnia magna of 10 -22 mg/L (based on water accommodated fractions).
Key value for chemical safety assessment
Additional information
One study report was available and input as an endpoint record for short term toxicity to aquatic invertebrates.
The study from Shell (1997) examined the short-term toxicity of the test substance Hydrocarbons C9-C12, n-alkanes, isoalkanes, cyclics, aromatics (2-25%) to Daphnia magna. 10 daphnia per test vessel (2 test vessels per concentration) were exposed to loading rates of 0, 1, 2.2, 4.6, 10, 22, 46, or 100 mg/l (WAF) of test substance. Daphnids were exposed for 48h, and there was no renewal of test solution. At 24 and 48 hrs, the Daphnia were observed for immobilization. Immobilization was seen at test concentrations of 10 mg/l (WAF) and above, with immobilization of all daphnids at the 46 and 100 mg/l concentrations by the 24 hr observation. The 48-hr EL50 range for an invertebrate, Daphnia magna, was 10-22 mg/L (WAF).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.