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EC number: 202-327-6 | CAS number: 94-36-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1979
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: 2d: Test procedure in accordance with national standard methods with acceptable restrictions (few datails in documentation)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Dibenzoyl peroxide
- EC Number:
- 202-327-6
- EC Name:
- Dibenzoyl peroxide
- Cas Number:
- 94-36-0
- Molecular formula:
- C14H10O4
- IUPAC Name:
- diphenylperoxyanhydride
- Details on test material:
- The test sample, a white granulated material, designated Lucidol and stated to consist of 75% di-benzoyl peroxide and 25% water, was received from the principal in November 1978. Appropriate solutions in acetone were prepared immediately before use.
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: S. typhimurium TA 1535, TA 1537, TA 98 , TA 100 and TA 1538
- Details on mammalian cell type (if applicable):
- The Salmonella typhimurium mutants were provided by Dr. B.N. Ames, Berkeley, California, USA and stored as frozen cultures at -80°C. To obtain fresh cultures for mutagenesis testing, nutrient broth is inoculated with the frozen culture and grown up overnight with shaking at 37°C.
The reversion properties of the strain were checked, using the mutagens 2-a+ninoanthracene and 9-aminoacridine.
In addition, the strains were checked Eor histidine requirements for sensitivity to crystal violet and deoxycholate.as well as for resistance to ampicilline. - Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- liver homogenate of Aroclor-induced rats (1254/kg ip, 7 days before sacrifice). The metabolic activation properties of the Iiver homogenate were checked with S. typh. TA 98 and 4-ABP.
- Test concentrations with justification for top dose:
- 0.8 up to 20-100, and 100-500 µg per plate
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene and 9 aminoacridine
- Statistics:
- not applicable
Results and discussion
Test results
- Species / strain:
- other: S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: only at highest levels (100-500 µg per plate)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- The present results did not reveal any mutagenic activity of the test material in the Salmonella/microsome mutagenicity test.
- Executive summary:
The potential of benzoyl peroxide to induce reverse mutation in Salmonella typhimurium (strains: TA 1535, TA 1537, TA 98, TA 100 and TA 1538) was evaluated in accordance with the Ames procedure, in presence and absence of metabolic activation. No data is available about compliance with the Principles of Good Laboratory Practice.
Incorporation of 0.8 up to 20 - 100 µg of the test material per plate did not induce an increase in the number of his+revertants with any of the five tester strains either in the presence or in the absence of S-9 mix. At higher levels, i.e. 100 - 50011g/plate,the testmaterial was toxic to the bacteria as seen by a less densebackground lawn of bacterial growth and a decrease in the number of his*revertants.From the present resuits it can be concluded thatbenzoyl peroxideat levels up to 20 - 100 ub/plate did notreveal any mutagenic activity in the plate incorporationassay.with S. typhimurium TA 1535, TA 1537, TA 1538, TA 98 or TA 100 in the presence or absence of the liver microsome activation system under the test conditions employed in this evaluation. At higher levels chemical toxicity interfered with the mutagenicity testing.
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