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Triacetin (CAS 102-76-1) is not considered to be harmful to aquatic organisms based on the results from short-term studies with fish, aquatic invertebrates and algae as well as long-term results on toxicity to aquatic invertebrates. No effects were observed up to a nominal concentration of 100 mg/L on aquatic invertebrates and algae with an LC50 (96 h) > 100 mg/L for Oryzias latipes and an EC50 (48 h) of 380 mg/L for Daphnia magna. The result with the freshwater algae Selenastrum capricornutum is in line with these results indicating low toxicity (ErC50 (72 h) > 940 mg/L and NOEC (72 h) 468 mg/L). An additional long-term study with Daphnia magna did not result in any toxic effects on reproduction and mortality after 21 d up to a nominal concentration of 100 mg/L (corresponding to a measured concentration of 94 mg/L). Triacetin did not cause inhibition of biodegradation in a study according to OECD 301B with activated sludge microorganisms. No inhibition of degradation was observed at the applied test concentration of 20 mg/L. Moreover, no effects on cell multiplication of Pseudomonas putida were observed up to a nominal test concentration of 1088 mg/L after 18 h. Thus, the overall potential to cause inhibition of activated sludge microorganisms is low and significant inhibition of the subsequent degradation process in sewage treatment plants is not expected.