2,2',2''-nitrilotriethanol

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1971

Report date:

1971

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Principles of method if other than guideline:

BASF-Test

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,2',2''-nitrilotriethanol

EC Number:

203-049-8

EC Name:

2,2',2''-nitrilotriethanol

Cas Number:

102-71-6

Molecular formula:

C6H15NO3

IUPAC Name:

2-[bis(2-hydroxyethyl)amino]ethan-1-ol

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: No. XXI/170

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Mean Weight at study initiation: 2.45 kg
- Diet: Altromin MS (Altroge, Germany) ad libitum
- Water: ad libitum

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: concurrent saline

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 50 m³
- Concentration: concentrated

Duration of treatment / exposure:

not applicable (substance was not washed out)

Observation period (in vivo):

8 days

Number of animals or in vitro replicates:

2 (m/f)

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing: none

Readings at 10 min, 1 and 24 hrs, 3 and 8 days

SCORING SYSTEM: initial scoring system was converted into Draize-scores

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

other: 1, 24 hours

Score:

1

Reversibility:

fully reversible within: 8 days

Remarks on result:

other: redness

Results 2

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

other: 8 days

Score:

0

Reversibility:

fully reversible within: 8 days

Remarks on result:

other: redness

Results 3

Irritation parameter:

chemosis score

Basis:

mean

Time point:

other: 1 hour

Score:

1

Reversibility:

fully reversible within: 8 days

Results 4

Irritation parameter:

chemosis score

Basis:

mean

Time point:

other: 24 hours, 8 days

Score:

0

Reversibility:

fully reversible within: 8 days

Any other information on results incl. tables

Readings	Animal	Cornea		Iris	Conjunctiva			Additional findings
		op	ar		red	ch	di
1 h	1	-	-	-	1	1	-
	2	-	-	-	1	1	-
24 h	1	-	-	-	1	0	-
	2	-	-	-	1	0	-
48 h	1
	2
72 h	1
	2
8 d	1	-	-	-	0	0	-
	2	-	-	-	0	0	-
Mean 24 -72 h	1
	2
Mean 24-72 h	1-2

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met