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EC number: 231-821-4 | CAS number: 7757-83-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Specific investigations: other studies
Administrative data
- Endpoint:
- specific investigations: other studies
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- other: not rated
- Rationale for reliability incl. deficiencies:
- other: In vitro study on effects of sodium sulfite on human neutrophils.
Data source
Reference
- Reference Type:
- publication
- Title:
- Functional responses of human neutrophils to sodium sulfite (Na2SO3) in vitro.
- Author:
- Labbé, P.; et al.
- Year:
- 1 998
- Bibliographic source:
- Human and Experimental Toxicolotgy, 17, 600-605
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The study focuses on the in vitro interactions of sodium sulfite (Na2SO3) with human neutrophils. The neutrophil suspension was isolated from the blood of healthy volunteers. It was used in concentrations of 0.1, 1 or 10 mM.
- GLP compliance:
- no
- Type of method:
- in vitro
- Endpoint addressed:
- immunotoxicity
Test material
- Reference substance name:
- Sodium sulphite
- EC Number:
- 231-821-4
- EC Name:
- Sodium sulphite
- Cas Number:
- 7757-83-7
- Molecular formula:
- NA2SO3
- IUPAC Name:
- disodium sulfate
- Details on test material:
- - Name of test material (as cited in study report): sodium sulfite
- Physical state: solid
Constituent 1
Results and discussion
- Details on results:
- Na2SO3 was not toxic to cells at 0.1, 1.0, or 10 mM (as assessed by Trypan blue exclusion.
Sodium sulfite induces superoxide production by itself and primes the response to fMLP:
- superoxide producton by neutriphils is increased by Na2SO3 in a concentration dependent fashion: after 5min, superoxide production from human neutrophils stimulated with buffer, 0.1, 1.0, 10 mM Na2SO3, or 10E-7 MPMA was 2.6±0.5 (mean±s.e.m., n=3); 4.5±1.3: 9.5±1.1; 22.3±2.1 and 24±4.0 mmoles/10E6 cells, respectively
- results were 3.3±0.1; 5.8±0.3; 14.0±0.1; 34.9±0.6 and 41.8±0.9 after 30 min of stimulation, in the same order
- Na2SO3 can also prime human neutrophils to respond to fMLP in a concentration-dependent fashion: after 30 min of incubation with buffer, 0.1, 1.0, 10 mM Na2SO3, or GM-CSF (positive control), superoxide production by fMLP-induced neutrophils (5 min) was 11.5±2.1; 14.5±2.1; 38.4±1.6 or 22.0±1.9 nmoles/10E6 cells
Na2SO3 induces RNA synthesis in a concentration-dependent fashion but does not induce cell shape changes:
- total RNA synthesis increased in a concentration dependent manner as assessed by incorporation of 5-[3H]uridine
- because of variability among individuals, results are expressed as stimulation indices and an index >1 indicates stimulation
- indices of stimulation were 1.01±0.08 (mean±s.e.m., n=8); 1.20±0.2; and 1.50±0.3 for 0.1, 1.0, or 10 mM Na2SO3, respectively and 4.93±0.7 for GM-CSF
- Na2SO3 did not induce neutrophil shape changes as the cells remained with a round morphology in contrast to the irregular morphology observed with GM-CSF
Na2SO3 does not modulate neutrophil apoptosis rate:
- neutrophil apoptosis rates remain similar to control cells (34.3±4.6 % of cells in apoptosis, mean ± s.e.m.) when incubated with 0.1 (41.5±9.3 %), 1.0 (33.2±5.3 %), or 10 mMNa2SO3 (44.7±6.1 %) in contrast to GM-CSF which, as expected, significantly delays neutrophil apoptosis (11.7±2.4 %)
- during co-incubation with GM-CSF, Na2SO3 did not reverse the known delaying effect of GM-CSF since the number of apoptotic cells was stable ranging from 15 to 17 %
Applicant's summary and conclusion
- Conclusions:
- The present study indicates that human neutrophils can be activated by Na2SO3. In particular, this sulfite strongly induces superoxide production by itself as well as it can prime neutrophils to respond to fMLP.
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