Registration Dossier
Registration Dossier
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EC number: 200-579-1 | CAS number: 64-18-6
Specific investigations: other studies
Administrative data
- Endpoint:
- specific investigations: other studies
- Adequacy of study:
- other information
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Abstract.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 002
Materials and methods
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Formic acid
- EC Number:
- 200-579-1
- EC Name:
- Formic acid
- Cas Number:
- 64-18-6
- Molecular formula:
- CH2O2
- IUPAC Name:
- formic acid
- Details on test material:
- TS-Freetext:
Sodium formate, formic acid.
Constituent 1
Results and discussion
Any other information on results incl. tables
Result: Cytotoxicity associated with undissociated formic acid
RS-Freetext:
The intracellular formate concentration was more significantly increased following treatment at pH 6.9 compared to the treatment at pH 7.4. Intracellular ATP was not changed at pH 7.4, but at pH 6.9. Similarly, viability was significantly decreased after 2 h at pH 6.9, but not at pH 7.4.
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Treatment
------------------------------------------------
Time 30 mM NaHCOO, pH 7.4 | 30 mM HCOOH, pH 6.9
-----------------------------------------------------------
Formate ATP | Formate ATP
(h) (µmol/mg protein) (%) | (µmol/mg protein) (%)
-----------------------------------------------------------
0 3 +/- 0.5 100 | 3 +/- 0.5 100
2 38 +/- 4 100 | 38 +/- 4 70
24 17 +/- 2 100 | 50 +/- 5 50
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Applicant's summary and conclusion
- Conclusions:
- CL-Freetext:
The authors concluded that cytotoxicity was pH-dependent. It was further concluded that the undissociated formic acid is the active inhibitor of mitochondrial cytochrome oxidase, and that formic acid is only permeable through the inner mitochondrial membrane it its undissociated form.
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