Kieselguhr, soda ash flux-calcined

Specific investigations: other studies

Administrative data

Endpoint:

cytotoxicity

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented and described

Data source

Reference

Reference Type:

publication

Title:

In vitro cytotoxicity and quantitative silica analysis of diatomaceous earth products

Author:

Bye E, Davies R, Griffiths DM, Gylseth B & Moncrieff CB

Year:

1984

Bibliographic source:

British Journal of Industrial Medicine 41: 228-234

Materials and methods

Principles of method if other than guideline:

Mouse peritoneal macrophages were used to evaluate the relative cytotoxicity of a series of diatomaceous earth products in vitro.

Type of method:

in vitro

Test material

Test material information

Constituent 1

Reference substance name:

Kieselguhr, soda ash flux-calcined

EC Number:

272-489-0

EC Name:

Kieselguhr, soda ash flux-calcined

Cas Number:

68855-54-9

Molecular formula:

SiO2

IUPAC Name:

silanedione

Details on test material:

Several diatomaceous earth products were examined, details are provided in table 1.

Results and discussion

Details on results:

The cytotoxicities of the high cristobalite content flux calcined materials were similar to that of the standard cristobalite; both the natural and straight calcined materials had significantly greater activities than the flux calcined materials. Thus within the limitations of the macrophage cytotoxicity test the hypothesis that the crytalline content is the only determinant of fibrogenicity of diatomaceous earth is not supported.