Silicic acid, aluminum sodium salt

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26 Oct. - 31 Oct. 1998

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water extract (water-accommodated fraction, WAF) by stirring corresponding suspensions for 24 h at 25 °C 
with subsequent filtration (paper) of the suspensions. Test solutions were then adjusted to pH 7.00.
- Differential loading: yes, 100, 1000, and 10,000 mg/L
- Controls: yes, untreated
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no data

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Strain: --
- Source: Aquafarm Ryba, D-27391 Zeven/Germany
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): ~3 cm
- Weight at study initiation (mean and range, SD): no data
- Method of breeding:
- Feeding during test: no


ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food:
- Feeding frequency:
- Health during acclimation (any mortality observed):


QUARANTINE (wild caught)
- Duration:
- Health/mortality:

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

none

Hardness:

no data

Test temperature:

24.9 - 25.1 °C

pH:

7.0 - 8.4

Dissolved oxygen:

7.2 - 8.0 mg O2/L

Salinity:

no data

Nominal and measured concentrations:

Loadings: 10,000 mg/L (main test)
0, 100, 1000, and 10,000 mg/L (range finding)

Details on test conditions:

TEST SYSTEM
- Test vessel: aquarium
- Type: open
- Material, size, headspace, fill volume: 5-L volume, no further data
- Aeration: yes
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: no data
- No. of animals per test concentration: 3 fish for 100 and 1000 mg/L, 7 fish for 10,000 mg/L
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates): not relevant
- Biomass loading rate: no data


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water (drinking water), corresponding to OECD-203 standard
no data available for the following parameters:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/Mg ratio:
- Conductivity:

- Culture medium different from test medium: no
- Intervals of water quality measurement: monthly

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: no data
- Light intensity: no data

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : behaviour, mortality


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Range finding study: conducted with the highest loading as limit test

Key result

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

>= 10 000 mg/L

Nominal / measured:

nominal

Remarks on result:

other: WAF of loading (filtered water extract)

Details on results:

No deaths occurred during testing. No particular findings.

Validity criteria fulfilled:

yes

Description of key information

No deaths occurred within 96 hours with the tested nanoform of Silicic acid, aluminum sodium salt (CAS 1344-00-9, NAS). The LC0 was equal or greater than 10000 mg/l after 96 h.

Additional information

It is assumed that the freshwater results are also applicable to marine life, i.e. no acute aquatic toxicity is expected from exposure to synthetic amorphous Silicic acid, aluminum sodium salt (CAS 1344-00-9, NAS).