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Studies on fish, daphnia and algae using excess loadings of NAS (sodium-aluminum silicate) or structure-analogous SAS (synthetic amorphous silicas [read-across] showed no acute toxicity; physical effects on the survival of daphniae were observed in tests using unfiltered test medium including suspended solids of SAS. Test results, based on loading rates, are as follows:

96h-LL0(Brachydanio rerio) = 10,000 mg/l for SAS and NAS;

24h-EL50 (Daphnia magna) >10,000 mg/l for SAS; 

72h-NOEL (Scenedesmus subspicatus) = 10,000 mg/l for NAS.

There are no chronic aquatic toxicity data, but due to the known inherent physico-chemical properties, absence of acute toxic effects as well as the ubiquitous presence of silica/silicates in the environment, there is no evidence of harmful long-term effects arising from exposure to synthetic amorphous silica/silicates.