4,4'-diaminostilbene-2,2'-disulphonic acid

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

non GLP, no analytical concentration verification

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

4,4'-diaminostilbene-2,2'-disulphonic acid

EC Number:

201-325-2

EC Name:

4,4'-diaminostilbene-2,2'-disulphonic acid

Cas Number:

81-11-8

Molecular formula:

C14H14N2O6S2

IUPAC Name:

2,2'-ethene-1,2-diylbis(5-aminobenzenesulfonic acid)

Details on test material:

- Name of test material (as cited in study report): 4,4'-diaminostilbene-2,2'-disulfonic acid
- Analytical purity: no data

Radiolabelling:

not specified

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Common carp
- Source: obtained from a commercial fish farm in Japan
- Length at study initiation (lenght definition, mean, range and SD): 10 cm
- Weight at study initiation (mean and range, SD): 30 g
- Lipid content of fish: 3.5 %

ACCLIMATION
- Acclimation conditions (same as test or not): flow through at 25 ± 2 °C
- Type and amount of food: obtained from a commercial fish farm in Japan at a rate of 2% of the total body weight
- Feeding frequency: daily, no feeding at the day of sampling

Study design

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

6 wk

Test conditions

Test temperature:

25 ± 2 °C

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: glass tanks, volum = 100 L
- Renewal rate of test solution (frequency/flow rate): 200 - 800 ml/min
- No. of vessels per concentration (replicates): 15 - 20
- No. of vessels per control / vehicle control (replicates): 15 - 20


TEST MEDIUM / WATER PARAMETERS
- Holding medium different from test medium: no
- Intervals of water quality measurement: twice a week

Nominal and measured concentrations:

Nominal: 0.1 and 1.0 mg/L

Results and discussion

Bioaccumulation factor

Bioaccumulation factor 1

Type:

BCF

Value:

< 0.5 dimensionless

Basis:

not specified

Remarks on result:

other: Conc.in environment / dose:1.0 mg/l

Bioaccumulation factor 2

Type:

BCF

Value:

< 4.7 dimensionless

Basis:

not specified

Remarks on result:

other: Conc.in environment / dose:0.1 mg/l

Applicant's summary and conclusion

Conclusions:

BCF < 0.5 (dimensionless)
BCF < 4.7 (dimensionless)

Executive summary:

BCF < 0.5 (dimensionless)
BCF < 4.7 (dimensionless)