Registration Dossier

Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions (non GLP, no analytical concnetration verification)

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1992
Report Date:
1992

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 4,4'-diaminostilbene-2,2'-disulfonic acid
- Analytical purity: no data
Radiolabelling:
not specified

Test organisms

Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: Common carp
- Source: obtained from a commercial fish farm in Japan
- Length at study initiation (lenght definition, mean, range and SD): 10 cm
- Weight at study initiation (mean and range, SD): 30 g
- Lipid content of fish: 3.5 %

ACCLIMATION
- Acclimation conditions (same as test or not): flow through at 25 ± 2 °C
- Type and amount of food: obtained from a commercial fish farm in Japan at a rate of 2% of the total body weight
- Feeding frequency: daily, no feeding at the day of sampling

Study design

Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
6 wk

Test conditions

Test temperature:
25 ± 2 °C
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: glass tanks, volum = 100 L
- Renewal rate of test solution (frequency/flow rate): 200 - 800 ml/min
- No. of vessels per concentration (replicates): 15 - 20
- No. of vessels per control / vehicle control (replicates): 15 - 20


TEST MEDIUM / WATER PARAMETERS
- Holding medium different from test medium: no
- Intervals of water quality measurement: twice a week
Nominal and measured concentrations:
Nominal: 0.1 and 1.0 mg/L

Results and discussion

Bioaccumulation factoropen allclose all
Type:
BCF
Value:
< 0.5 dimensionless
Basis:
not specified
Remarks on result:
other: Conc.in environment / dose:1.0 mg/l
Type:
BCF
Value:
< 4.7 dimensionless
Basis:
not specified
Remarks on result:
other: Conc.in environment / dose:0.1 mg/l

Applicant's summary and conclusion