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EC number: 213-561-3 | CAS number: 980-26-7
C.I. Pigment Red 122
mean of living offspring
coefficient of variation
C.l. Pigment Red 122 was tested in Daphnia magna (water flea) over 21 days in a semistatic system. Since no effects were expected up to the limit of solubility a limit test with a nominal concentration of 1 mg/L was conducted.
A negative control (0 mg/L) and a nominal concentration of 1 mg/L were tested. The tested concentration is already above the limit of solubility of the test substance in water (¿ 20 µg/L).
The test was conducted under semistatic conditions. The media and the test solutions were renewed every Monday, Wednesday and Friday. For every renewal new test solutions were prepared. This was done by stirring the test substance for 48 hours into the test medium. The aqueous phase of the concentration group was present as a slight reddish clear solution during the entire exposure period. There were no visible particulate matters. Water parameters were within the normal range.
Analytical monitoring of the concentration of the test substance in the test water was not conducted since no relevant results could be expected. The solubility limit of the test substance is below the analytical detection limit. From the properties of the test substance it could be concluded that the test substance is chemically stable in water over the test intervals of 48 and 72 hours, respectively.
The concentration values given in this report are based on the nominal concentration.
In the control group no mortality occurred and the mean number of living offspring produced per parent animal was 102.2, thus fulfilling the validity criteria for the test.
No immobility occurred in the 1 mg/L (nominal concentration) and in the control group. No difference in the onset of brood production was observed in the concentration group in comparison to the control. The reproduction rate in the concentration group showed no statistically significant changes in comparison to the control.
In this prolonged toxicity study with C.I. Pigment Red 122 in Daphnia magna over 21 days the following effect concentrations were obtained:
NOEC (immobility): > 1 mg/L (nominal concentration), > solubility limit (¿ 20 µg/L)
NOEC (reproduction rate): > 1 mg/L (nominal concentration), > solubility limit (¿ 20 µg/L)
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