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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP report with certificate conducted at highly qualified laboratory.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report Date:
1989

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
not specified
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Sampled prior to study and at study termination (two times). Flasks were sealed during the study to prevent loss of test material.

Test solutions

Vehicle:
no
Details on test solutions:
Flasks were sealed during the study to prevent loss of test material.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Groups of 10 Daphnid less than 24-hrs old were tested in separate flasks. A total of 8 exposure levels (including a control) were evaluated in duplicate.

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Post exposure observation period:
none

Test conditions

Hardness:
Total alkalinity 84; EDTA hardness 77; Conductivity 160
Test temperature:
19.9 to 20.0 deg C
pH:
7.2 to 7.6
Dissolved oxygen:
reported to be below 40% saturation at 390 mg/l but with in acceptable range for all other exposure levels. at 390 mg/l 2.6 to 4.9 mg/l; at 62 mg/l 7.5 to 7.8 mg/l; at 13 mg/l 8.3 to 8.4 mg/l control 8.4 to 8.5 mg/l
Salinity:
data not available
Nominal and measured concentrations:
Nominal concentration(mg/l) 0(control), 16, 27, 45, 76, 144, 210, 350 analytical concentration(mg/l) 0(control) 13, 23, 31, 62, 140, 210, 390
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
other: 80% immobility
Effect conc.:
390 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

The 48 -hr EC50 for daphnia magna was calculated to be 300 mg/l with 95% confidence limits of 260 to 350 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
EC50 for daphnia magna was calculated to be 300 mg/l with 95% confidence limits of 260 to 350 mg/l under static conditions at 20 deg. C.
Executive summary:

EC50 for daphnia magna was calculated to be 300 mg/l with 95% confidence limits of 260 to 350 mg/l.under static conditions at 20 deg. C. The mean measured concentrations in the test flasks ranged from 81 to 111% of the nominal concentrations.