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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP laboratory study from original report

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report Date:
1989

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
Concentrations of test substance in solution were o (control), 1.3, 2.5, 6.4, 9.5, 22, and 40 mg/l.

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
Groups of 10 unsexed trout approx. 3.3 cm in length and 0.526 gms.

Study design

Test type:
flow-through
Water media type:
freshwater
Total exposure duration:
96 h
Post exposure observation period:
none

Test conditions

Hardness:
data not available, water obtained from a 360 well
Test temperature:
12.0 to 12.3 deg C
pH:
data not available
Dissolved oxygen:
assumed to be at or near saturation. Air bubbled continuously through the water
Salinity:
data not available
Nominal and measured concentrations:
Nominal Concentrations (mg/l) 0(control), 11, 22, 44, 88, 175, and 350 while the Average Measured concentrations were (mg/l) 0.3, 1.3, 2.5, 6.4, 9.5, 22, and 40, respectively. This large difference between nominal concentrations and measured concentrations is the result of testing a highly volatile material (boiling point -47.4 deg C) that is only slightly soluble in an aqueous system.
Details on test conditions:
The large differences between nominal and measured concentrations result from losses of test substance due to the high volatility (boiling point -47.4 deg C) and relatively low solubility in water.
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC10
Effect conc.:
ca. 40 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks:
1 fish died at 72 hrs
Details on results:
The large difference between nominal concentrations and measured concentrations is the result of testing a highly volatile material (boiling point -47.4 deg C) that is only slightly soluble in an aqueous system.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 96-hr LC50 in rainbow trout was determined to be > 40 mg/l (analytically measured by GC) The nominal concentration for this level was 350 mg/l. This large difference between nominal concentrations and measured concentrations is the result of testing a highly volatile material (boiling point -47.4 deg C) that is only slightly soluble in an aqueous system and rapidly is lost from the test system. It is therefore concluded that the calculated value of 109 mg/L is the more accurate value.
Executive summary:

The 96-hr LC50 in rainbow trout was determined to be > 40 mg/l (analytically measured by GC) The nominal concentration for this level was 350 mg/l. This large difference between nominal concentrations and measured concentrations is the result of testing a highly volatile material (boiling point -47.4 deg C) that is only slightly soluble in an aqueous system and rapidly is lost from the test system. It is therefore concluded that the calculated value of 109 mg/L is the more accurate value.