Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 500-130-2 | CAS number: 55818-57-0
Table 1: Micronucleus Assay - Summary Table
Harvest Time (hours)
% Micronucleated PCEs
Mean of 2000 per
Animal ± S.E.(Males)
Mean ± S.E.(Males)
Corn Oil 20 mL/kg
0.04 ± 0.02
0.51 ± 0.06
0.03 ± 0.02
0.35 ± 0.01
CP 80 mg/kg
2.77 ± 0.28*
0.55 ± 0.1
0.04 ± 0.03
0.43 ± 0.05
0.06 ± 0.02
0.32 ± 0.07**
0.02 ± 0.01
0.59 ± 0.06
0.05 ± 0.02
0.4 ± 0.08
* Significantly greater than the corresponding vehicle control, p = 0.01.
** Significantly less than the corresponding vehicle control, p = 0.05.
CP = Cyclophosphamide; PCE = Polychromatic erythrocyte; NCE = Normochromatic erythrocyte
a Due to solubility limit, the high dose was dosed at 1000 mg/kg BID (~1 hr apart) to achieve a dose of 2000 mg/kg.
In a bone marrow micronucleus test, performed according to OECD guideline 474, in compliance with GLP, CD-1® (ICR) BR male mice (5/dose) were given a single oral (gavage) dose of Bisphenol A diglycidyl ether diacrylate (BADGE Diacrylate) in corn oil at concentrations of 500 and1000 mg/kg bw and twice, approximately one hour apart, at concentration of 2000 mg/kg bw (1000 mg/kg/dose, BID). Bone marrow was extracted after 24 or 48 hours of exposure and the prepared slides were scanned to determine the frequency of micronuclei in 2000 polychromatic erythrocytes (PCEs) per animal. In addition, the number of PCEs and normochromatic erythrocytes (NCEs) in a population of 500 erythrocytes was determined as a measure of cytotoxicity. A preliminary range-finding test was also conducted on 3 mice/sex/dose at 500, 1000 and 2000 mg/kg bw and animals were observed for 2 days. In this previous test, no mortality and/or toxic signs were recorded.
No statistically significant increases in the frequency of micronucleated PCEs were observed at any dose levels. A statistically significant decrease in the PCE:NCE ratios at a dose level of 1000 mg/kg bw was observed but was not considered biologically significant as the highest dose of 2000 mg/kg bw did not show a similar trend.
Under the test conditions, BADGEDA is not considered as mutagenic in the mouse bone marrow micronucleus test according to the criteria of the Annex VI to the Directive 67/548/EEC and CLP Regulation (EC) N° (1272-2008).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Close Do not show this message again