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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Meets national standard method with acceptable restrictions, well documented

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Unnamed
Year:
1977
Reference Type:
publication
Title:
Unnamed
Year:
1980
Report Date:
1980

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: DIN 38412, part 8 (German Standard Method)
Principles of method if other than guideline:
Cell proliferation inhibition test, measurement of the turbidity at 436 nm.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
No data
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
Not applicable

Test solutions

Vehicle:
no
Details on test solutions:
No data

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
Laboratory culture

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
16 h
Post exposure observation period:
Not applicable

Test conditions

Hardness:
No data
Test temperature:
25ºC
pH:
No data
Dissolved oxygen:
No data
Salinity:
Not applicable
Nominal and measured concentrations:
Nominal concentrations not reported.
Details on test conditions:
No data
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Duration:
16 h
Dose descriptor:
other: Toxic Threshold Concentration, MIC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Details on results:
Cell proliferation inhibition: the toxic threshold concentration (=TGK) is equivalent to the minimal inhibitory concentration (MIC) and is defined as the concentration with incipient decrease in the extinction value (>=3%) at the end of the test period.

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
In a standard bacterial test on cellular proliferation using the bacteria Pseudomonas putida (according to DIN 38412, part 8), no significant inhibitory effect was observed up to 100 mg/L of butyraldehyde following exposure for 16 hours.
Executive summary:

In a standard bacterial test on cellular proliferation using the bacteria Pseudomonas putida (according to DIN 38412, part 8), no significant inhibitory effect was observed up to 100 mg/L of butyraldehyde following exposure for 16 hours.