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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Basic data given; comparable to guideline study, national standard method. Only 24h exposure but 48 h are not expected to make EC50 fall below 100 mg/L.
Cross-reference
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1982
Report Date:
1982

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: DIN 38412, part 11
Principles of method if other than guideline:
guideline DIN 38412, part 11: Determination of the effect of water ingredients to small crustaceans (acute Daphnia-Test)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Not applicable
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Strain: Daphnia magna Straus IRCHA
- Source: inhouse brood
- Age at study initiation (mean and range, SD): =<24 h
- Method of breeding: continuous
- Feeding during test
- Food type: Algae as standardised dry feed (from Dohse Aquaristik9



Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h

Test conditions

Test temperature:
20°C
pH:
8.0 +/- 0.2 (test start)
Dissolved oxygen:
>/= 2 mg/L (test end)
Salinity:
Not applicable
Nominal and measured concentrations:
Nominal concentrations not reported.
Details on test conditions:
Test container volume: 50 mL;
test container: glass beakers;
test liquid volume: 20 mL
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
100 mg/L
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
195 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 160-238 mg/L
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
383 mg/L
Results with reference substance (positive control):
EC50 (K-dichromate) = 1.3 mg/L [range in which it should be (recommended by literature): 0.9 -1.9 mg/L]
Reported statistics and error estimates:
graphical EC50 determination

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The EC50 of butyraldehyde under these conditions was relatively high with 195 mg/L, the EC0 was 100 mg/L, while the EC100 was almost 400 mg/L. Overall, the dose-response curve was relatively smooth and not steep.
Executive summary:

In an early comprehensive test programme on acute aquatic toxicity, the German standard method DIN 38412, part 11 [Determination of the effect of water ingredients to small crustaceans (acute Daphnia-Test)], was employed. The EC50 of butyraldehyde under these conditions was relatively high with 195 mg/L, the EC0 was 100 mg/L, while the EC100 was almost 400 mg/L. Overall, the dose-response curve was relatively smooth and not steep.