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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
96 hours
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to common international guidelines (e.g. OECD 203); meets national standard method with acceptable restrictions, basic data given.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1981

Materials and methods

Test guideline
Guideline:
other: EPA-660/3-75-009
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
No other details given
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
analytical samples at test start (0h) and test end (96h)

Test solutions

Vehicle:
no
Details on test solutions:
Test media were prepared either bu direct addition to the test vessels or in the form of a stock solution in deionised water. The solutions were briefly stirred with a glass rod.

Test organisms

Test organisms (species):
Pimephales promelas
Details on test organisms:
use of controlled breeding stocks provided by E.P.A. Newtown Fish Toxicology Station, Cincinnati, Ohio;
pre-test observation period: at least 14 days;
only use of healthy populations: mortality in pre-test observation period not more than 3%;
acclimation period (to test water): 4 days (i.e. 2 days transition followed by 2 days in 100% test medium)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
Not applicable

Test conditions

Hardness:
40-48 mg/L (as CaCO3)
Test temperature:
22 +- 1°C (test containers were placed in a large circulating temperature bath)
pH:
7.2-7.9
Dissolved oxygen:
monitored once daily
Salinity:
Not applicable
Nominal and measured concentrations:
Range of concentrations tested and analytical results not reported.
Details on test conditions:
test volume: 12 L (with a heigth of ca. 24 cm);
replicates per treatment group: 2;
test animal per replicate: 5;
total amount of animals per treatment group: 10;
two control replicates were each containing five fish were also used;
amount of test item concentrations tested (inclusive the control): at least 6;
geometric series between the test item concentrations: 0.6;
validity criteria for control mortality: <10%;
determinations every 24h: survival, dissolved oxygen, temperature;
determinations at test end: pH, weighing of control animals (to determine average size of test organisms)
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
25.8 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% CL: 19.3-33.6 mg/L; 25.8
Results with reference substance (positive control):
Not applicable
Reported statistics and error estimates:
Statistical evaluation of results: determination of 96h-LC50 and 95% confidence limits (either by probit, moving average, or binomial test, depending on toxicity profile of the test item)

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Remarks:
analytical data on stability not specified
Conclusions:
The LC50 value for n-butanal is reported to be 25.8 mg/L (with 95 % confidence limits of 19.3 - 33.6 mg/L).
Executive summary:

Within a comprehensive screening programme for establishing national water quality standards (US EPA), 42 chemicals were screened for acute aquatic toxicity in the 96h standard test (US national standard) using the fathead minnow. The LC50 value for n-butanal is reported to be 25.8 mg/L (with 95 % confidence limits of 19.3 - 33.6 mg/L).