Methylamine

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented publication which meets basic scientific principles

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

not specified

GLP compliance:

no

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

Methylamine

EC Number:

200-820-0

EC Name:

Methylamine

Cas Number:

74-89-5

Molecular formula:

CH5N

IUPAC Name:

methanamine

Details on test material:

liquid methylamine

Test animals

Species:

guinea pig

Strain:

Hartley

Sex:

female

Administration / exposure

Type of coverage:

open

Vehicle:

other: acetone and dry ice

Duration of exposure:

the effects were recorded direct after drops fallen on the skin

Doses:

two or three drops

No. of animals per sex per dose:

3

Control animals:

yes, concurrent vehicle

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

other: substance is corrosive

Effect level:

other: drops of undiluted test material

Any other information on results incl. tables

On contact with the skin, the excess base rapidly vaporized leaving no surface residue. However, immediately following this exposure, the skin changed colour and within a few minutes it was swollen and suffused with a dull purple hue. At 48 h the areas of application were gray; histologically, at that time the test skin was necrotic: the epidermis was identified only by keratinous debris; in the dermis, the collagen was disorganized, and there was haemorrhage and a massive infiltrate of round cells and polymorphonuclear leucocytes. At 12 days, the dermis consisted of fresh granulation tissue over which there extended a relatively flat epidermis; there were no hair follicle. The experimantal guinea pigs appeared in a good general health during this time.

Applicant's summary and conclusion

Interpretation of results:

other: corrosive if liquified

Remarks:

Criteria used for interpretation of results: expert judgment

Conclusions:

Methylamine is clearly a powerful primary cutaneous irritant. Readily penetrate stratum corneum.

Executive summary:

In the acute toxicity by dermal application experiments performed by Goffmann and Maguire in 1980, three guinea pigs were used to assess dermal toxicity of liquefied methylamine. The animals received two to three drops of the liquefied gas unchanged onto the skin, and the skin reactions were observed for a period of 12 days. The clinical signs were immediate skin color change, and swelling with subsequent necrosis. Histology performed revealed after 48 h: necrotic skin, and after 12 days: fresh granulation tissue. So the test material caused skin necrosis.