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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented publication which meets basic scientific principles

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1980

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
Deviations:
not specified
GLP compliance:
no
Test type:
standard acute method

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
liquid methylamine

Test animals

Species:
guinea pig
Strain:
Hartley
Sex:
female

Administration / exposure

Type of coverage:
open
Vehicle:
other: acetone and dry ice
Duration of exposure:
the effects were recorded direct after drops fallen on the skin
Doses:
two or three drops
No. of animals per sex per dose:
3
Control animals:
yes, concurrent vehicle

Results and discussion

Effect levels
Sex:
female
Dose descriptor:
other: substance is corrosive
Effect level:
other: drops of undiluted test material

Any other information on results incl. tables

On contact with the skin, the excess base rapidly vaporized leaving no surface residue. However, immediately following this exposure, the skin changed colour and within a few minutes it was swollen and suffused with a dull purple hue. At 48 h the areas of application were gray; histologically, at that time the test skin was necrotic: the epidermis was identified only by keratinous debris; in the dermis, the collagen was disorganized, and there was haemorrhage and a massive infiltrate of round cells and polymorphonuclear leucocytes. At 12 days, the dermis consisted of fresh granulation tissue over which there extended a relatively flat epidermis; there were no hair follicle. The experimantal guinea pigs appeared in a good general health during this time.

Applicant's summary and conclusion

Interpretation of results:
other: corrosive if liquified
Remarks:
Criteria used for interpretation of results: expert judgment
Conclusions:
Methylamine is clearly a powerful primary cutaneous irritant. Readily penetrate stratum corneum.
Executive summary:

In the acute toxicity by dermal application experiments performed by Goffmann and Maguire in 1980, three guinea pigs were used to assess dermal toxicity of liquefied methylamine. The animals received two to three drops of the liquefied gas unchanged onto the skin, and the skin reactions were observed for a period of 12 days. The clinical signs were immediate skin color change, and swelling with subsequent necrosis. Histology performed revealed after 48 h: necrotic skin, and after 12 days: fresh granulation tissue. So the test material caused skin necrosis.