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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure according to national standards (DIN), no GLP compliance.
Qualifier:
according to
Guideline:
other: DIN 38412, part 11
GLP compliance:
no
Analytical monitoring:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
Test organisms: Daphnia magna 6 to 24 hours old derived from inhouse cultures.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
Total hardness: 2.4 mM
Ca/Mg-ratio: 4:1
Na/K-ratio: 10:1
Test temperature:
Test solution kept at 20 °C
pH:
Initial pH: 8.0 ± 0.2
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
95 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
(not neutralized)
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
163 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
(not neutralized)
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
260 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
(not neutralized)
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
702 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
(neutralized)
Basis for effect:
mobility
Validity criteria fulfilled:
yes
Conclusions:
After a duration of 48 hours an EC0 of 95 mg/L, EC50 of 163 mg/L, and EC100 of 260 mg/L, based on not neutralized test material concnetrations, were reported. An LC50 of 702 mg/L is also given, based on neutralized test material concentration. The basis for effect was the mobility of the animals.
Executive summary:

In 1989, Kuehn R. et.al. published a study for the short - term toxicity of Daphnia magna to monomethylamine. The experiment was performed in accordance to DIN 38412, part 11. In a static test with freshwater the following results were shown:

EC0 of 95 mg/L, EC50 of 163 mg/L, and EC100 of 260 mg/L, based on not neutralized test material concentrations. An LC50 of 702 mg/L is also given, based on neutralized test material concentration. The basis for effect was the mobility of the animals.

Description of key information

The EC50 of 163 mg/L was chosen as critical value for the chemical safety assessment due to the fact that the time duration of the test is given in the key study. (No time duration is given in the available supporting study.)

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
163 mg/L

Additional information

In 1989, Kuehn R. et.al. published a study for the short - term toxicity of Daphnia magna to monomethylamine. The experiment was performed in accordance to DIN 38412, part 11. In a static test with freshwater the following results were shown:

EC0 of 95 mg/L, EC50 of 163 mg/L, and EC100 of 260 mg/L, based on not neutralized test material concentrations. An LC50 of 702 mg/L is also given, based on neutralized test material concentration. The basis for effect was the mobility of the animals.

For short - term toxicity a reliable handbook data is available were a result of EC50 = 480 mg/L is given for Daphnia magna. Unfortunately, no exposure time is included. However, it is a reliable handbook data.