Registration Dossier
Registration Dossier
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EC number: 215-146-2 | CAS number: 1306-19-0
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Remarks:
- Type of genotoxicity: other: chromosome aberration and sister chromatid exchanges
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- not applicable
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Reliable with restriction. Not according to GLP nor to specific test guidelines.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- publication
- Title:
- Effect of fetal calf serum on the cadmium clastogenicity
- Author:
- Wang TC and Lee ML
- Year:
- 2 001
- Bibliographic source:
- Mutat Res 498: 79-87
Materials and methods
- Principles of method if other than guideline:
- CA:
-incubation time: 20 hours
-number of cells observed: 100 metaphases per culture
-staining: 3% Giemsa
SCE:
-incubation time: 24 hours
-number of cells observed: 50 metaphases per culture
-staining: fluorescence dye 33258 Hoechst plus Giemsa - GLP compliance:
- not specified
- Type of assay:
- other: in vitro mammalian chromosome aberrations test and sister chromatid exchange assay in mammalian cells
Test material
- Reference substance name:
- Cadmium carbonate
- EC Number:
- 208-168-9
- EC Name:
- Cadmium carbonate
- Cas Number:
- 513-78-0
- IUPAC Name:
- cadmium carbonate
- Details on test material:
- name of test substance: cadmium carbonate (FERAK, Berlin, Germany)
Constituent 1
Method
- Target gene:
- not applicable
Species / strain
- Species / strain / cell type:
- Chinese hamster Ovary (CHO)
- Details on mammalian cell type (if applicable):
- - Type and identity of media: McCoy 5A media + 10%FCS, sodium bicarbonate and antibiotics
- Additional strain / cell type characteristics:
- other: CHO cells were recloned for its karyotype stability and designated as CHO-W8 strain
- Metabolic activation:
- not applicable
- Metabolic activation system:
- not applicable
- Test concentrations with justification for top dose:
- CA: 0, 12.5, 25.0, 50.0, 100.0 µM
SCE: 0, 13.0, 25.0, 50.0, 100.0 µM - Vehicle / solvent:
- H2O
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- no
- Positive control substance:
- no
- Remarks:
- none
- Details on test system and experimental conditions:
- none
- Evaluation criteria:
- no information
- Statistics:
- not reported
Results and discussion
Test results
- Species / strain:
- Chinese hamster Ovary (CHO)
- Metabolic activation:
- not applicable
- Genotoxicity:
- other: CA (positive) and SCE (negative)
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- not specified
- Additional information on results:
- none
- Remarks on result:
- other: strain/cell type: CHO cells
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
none
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
other: CA: positive and SCE: negative
This study reported that CA in Chinese hamster ovary (CHO-W8) were observed following cadmium carbonate treatment. Cadmium carbonate did not induce significant SCE at the same dose level that yielded significant chromosome aberrations. - Executive summary:
Wang and Lee (2001) treated Chinese hamster ovary (CHO-W8) cells with cadmium carbonate and found no induction of
SCE at the same dose level that yielded significant chromosome aberrations.
In this study 6 cadmium compounds were tested: cadmium acetate, cadmium carbonate, cadmium chloride, cadmium nitrate, cadmium oxide and cadmium sulfate. Cadmium nitrate, carbonate and oxide induced significant chromosome aberration, while the other three cadmium compounds gave negative results. All 6 cadmium compounds did not induce significant SCE
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