1,1'-(ethane-1,2-diyl)bis[pentabromobenzene]

Toxicity to soil microorganisms

Administrative data

Endpoint:

toxicity to soil microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Jan 14 - June 9, 2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: study performed according to international guidelines and GLPs using the commercial product as test article and published in the literature.

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

2008

Reference 2

Reference Type:

publication

Title:

Terrestrial toxicity evaluation of decabromodiphenyl ethane on organisms from three trophic levels.

Author:

Hardy et al.

Year:

2011

Bibliographic source:

Ecotoxicol Environ Saf 74(4):703-10.

Reference 3

Reference Type:

publication

Title:

Results of terrestrial and aquatic studies on the brominated flame retardant, decabromodiphenyl ethane, a.k.a., Ethane 1,2 bis(pentabromophenyl)

Author:

Hardy et al.

Year:

2009

Bibliographic source:

SETAC Annual Meeting, Nov 2009, New Orleans, LA

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,1'-(ethane-1,2-diyl)bis[pentabromobenzene]

EC Number:

284-366-9

EC Name:

1,1'-(ethane-1,2-diyl)bis[pentabromobenzene]

Cas Number:

84852-53-9

Molecular formula:

C14H4Br10

IUPAC Name:

1,2,3,4,5-pentabromo-6-[2-(2,3,4,5,6-pentabromophenyl)ethyl]benzene

Details on test material:

The test article was 97.64% DBDPEthane.

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

no

Test organisms

Test organisms (inoculum):

soil

Study design

Total exposure duration:

28 d

Test conditions

Test temperature:

18.1 to 20.8 degrees C

Moisture:

43.0-54.1% of Water Holding Capcity

Nominal and measured concentrations:

0, 2500 mg DBDPEthane/kg dry soil, 500 mg positive control/kg dry soil

Reference substance (positive control):

yes

Remarks:

2 -chloro-6 -(trichloromethyl) pyridine

Results and discussion

Effect concentrations

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

2 500 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

nitrate formation rate

Details on results:

The mean nitrate formation rates were 6.1 mg No3/kg dry soil/d in untreated controls, 5.8 mg/kg/d in soils treated with DBDPEthane adn 0.3 mg/kg/d in soils treated with the positive control substances. Soils treated with 2500 mg/kg DBDPEthane showed 5.9% inhibtion compared with untreated controls. Soils treated with 500 mg/kg positive control substance showed 95.0% inhibition in the rate of nitrate formation compared to controls.

Results with reference substance (positive control):

The mean nitrate formation rates were 6.1 mg No3/kg dry soil/d in untreated controls, 5.8 mg/kg/d in soils treated with DBDPEthane adn 0.3 mg/kg/d in soils treated with the positive control substances. Soils treated with 2500 mg/kg DBDPEthane showed 5.9% inhibtion compared with untreated controls. Soils treated with 500 mg/kg positive control substance showed 95.0% inhibition in the rate of nitrate formation compared to controls.

Any other information on results incl. tables

Treatment	Soil Nitrate (mg NO3/kg dry soil)
	Day 0	Day 28	Inhibition (%)
Control	26.9	199	---
DBDPEthane (2500 mg/kg dry soil)	26.0	188	5.6
Reference Inhibitor (500 mg/kg dry soil)	24.3	32.8	83.5

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

DBDPEthane did not have any long-term adverse effects on soil nitrogen transformation.

Executive summary:

Soil treated with DBDPEthane at a nominal concentration of 2500 mg/kg exhibited approximately 6% inhibition in the rate of nitrate formation after 28 days, compared with untreated control soil; therefore, the test substance did not have any long-term adverse effects on nitrogen transformation.